Antioxidant
abstract
  Traditional Chinese herbs are widely used in our daily lives. But many of their effects are still unknown in science studies. My lab's purpose is to prove Chinese herbs' effect in medicine. Last two months, I used vitro assay to demonstrate 金線連 and 五種台灣本土菊科植物 have significant effects of antioxidant. It is so amaizing. We will work for vivo assay to make sure the effects in future.

introduction

     Free Radial Introduction
     Flavonoids as Antioxidant
     Flavonoid glucosides act less effectively..
Meterials & Methods
     DPPH assay
     Deoxyribose Method
     DNA protect
     Brain Lipid
     NBT assay
     hemolysis assay
Result & Discussion
     金線蓮
     五種本土菊科植物
References

1.Toren Finkel & Nikki J. Holbrook.(2000) Oxidants, osidative stress and the biology of ageing. Nature, VOL408, 239-247.
2. Pier-Giorgio Pietta, Flavonoids as antioxidant. J. Nat. Prod. 2000,63, 1035-1042.
3.Kana Ioku, Tojiro Tsushida, Yoko Takei, Nobuji Nakatani, and Junji Terao. Antioxidative activity of quercetin and quercetin monoglucoside in solution and phospholipid biolayers. Biochimica et Biophysica Acta 1234(1995) 99-104
4.Chun Hu and David D. Kitts. Studies on the Antioxidant Activity of Echinacea Root Extract. J. Agric. Food Chem 2000, 48, 1466-1472.
5.F. Liu and T.B.Ng. Antioxidative and free radical scavenging activities of selected medicinal herbs. Life Sciences, Vol 66, NO. 8, pp.725-735, 2000.
6.Barry Halliwell, John M. C. Gutteridge, and Okezie I. Aruoma. The Deoxyribose Method: A Simple "Test-Tube" Assay for Determination of Rate Constants for Reactions of Hydroxyl Radials. Analytical Biochemistry 165, 215-219(1987)
7.Gow-Chin Yen and Chiu-Luan Hsieh. Antioxidant Activity of Extracts from Du-zhong(Eucommia ulmoides) toward Various Lipid Peroxidation Models in vitro. J. Agric. Food Chem 1998, 46, 3952-3957.
8. Yoko Aniya, Takahiro Yokomakura, Masato Yonamine, Katsumasa Shimada, Tatsumi Nagamine, Megumi Shimabukuro, Hisako Gibo. Screening of antioxidant action of various molds and protection of Monascus anka against experimentally induced liver injuries of rats. General Pharmacology 32(1999) 225~231.
9.Young-Sam Keum, Kwang-Kyun Park, Jong-Min Lee, Kyung-Soo Chun, Jeong Hill Park, Seung Ki Lee, Hoonjeong Kwon, Young-Joon Surh. Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Letters 150(2000) 41-48.