¡@¡@Blood was obtained from sheeps and collected in tubes. Erythrocyte were seperated from plasma and the buffy coat and wathed three times with 9 mg/ml NaCl, and two times with PBS 1x buffer. Then dilute it to 10% suspesion in PBS buffer(phosphate buffered saline , pH 7.4).
¡@¡@Added 10% suspension of erythrocyte 0.8ml and 200mM AAPH(in PBS) 0.4mM, and incubate in 37¢J for 2 hours. The reaction mixture was then removed, diluted with 8 volumes of PBS and centrifuged at 2500rpm for 10 min. The absorbance A of the supernatane was read at 540 nm