¡@¡@This expriment depends on some equations. When hydroxyl radial attack deoxyribose and lipid, Deoxyribose and lipid will fragmentize to MDA.
¡@¡@MDA then will binds TBA(2-thiobarbituric acid) to form pink MDA-TBA chromogen. This chromogen will absorb at 532 nm

¡@¡@For the in vitro studies, the brains of normal rats were disseccted and homogenized in 20mM, pH=7.4 Tris-HCl buffer(brain:buffer=1:9). The homogenate was centrifuged at 14000rpm for 15min. 1ml of supernatant were incubated with the test samples in the presence of 10 £gM FeSO4 and 0.1mM ascorbic acid at 37¢J for 1 hour. Then add 1ml TCA(trichoroacetic acid, 28%, w/v) and 1.5ml TBA(thiobarbituric acid, 1%, w/v), boiling it at 100¢J for 15 min. After centrifugation and remove precipitated substrates. Detected pink complex at 532 nm.