Construction of pEGFP(-N3)5E2HA Vector: pEGFP-N3 is cut by AflI Use: Fusion to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo. Insert: A fragment containing Pcmv promoter-E2-HA is cut from pC5E2HA by SalI Further treatment of vector & insert: 1. vector(cut) ¡÷ Fill-in ¡÷ CIP 2. insert ¡÷ Fill-in Ligation Transformto abstract