Construction of pEGFP(-N3)5E2HA
                     
Vector:  pEGFP-N3 is cut by AflI 
    Use: Fusion to the N terminus of EGFP retain the fluorescent properties 
     of the native protein allowing the localization of the  fusion protein in vivo.
         
 Insert:  A fragment containing Pcmv promoter-E2-HA 
               is cut from pC5E2HA  by SalI

 Further treatment of  vector & insert:

   1. vector(cut)  →  Fill-in  →   CIP    
                                                                            
    2. insert  →  Fill-in 
      
  Ligation
          
 Transform

                  to abstract