Fill-in condition 10¡Ñfill-in buffer 3(£gL) 20mM DTT 1.5 0.5mM dNTP 3 50£gg/mL BSA 1.5 DNA 18 H2O 2 total 29 method 1¢X solution(29£gL) ¡÷ 37¢XC water bath 5min 2¢X room temp. 5 min 3¢X add 1£gL enzyme (Klenow:large fragement of E. coli polymerase I) 4¢X room temp. 30 min