Ligation Vector(CIP) and insert (fill-in) are purification by phenol/chloroform condition 1. reaction solution 10¡Ñ buffer 1 PEG4000 1 T4 ligase 1 total 3
3 reactions |
vectors |
inserts |
H2O |
reaction solution |
total |
control |
2 |
0 |
5 |
3 |
10 |
ligation-1 |
2 |
2 |
3 |
3 |
10 |
ligation-2 |
2 |
4 |
1 |
3 |
10 |
2.incubate the 3 reaction in 22¢XC water bath for 2 hours.