HOMEWORK#5

Sequence comparison / Homology search

• due on 12/17

• Shirley Lee, a student in department of Life Sciences, was bitten by a snake in the backyard of life science building. She was so angry! Thus, she killed the snake and purified several toxins from its venoms. She sequenced one of the toxin, got this sequence:

  1 L K C N K L V P L F Y K T C P A G K N I C Y K M F M V A T P

  31 K L P V K R G C I D V C P K S S L L V R Y V C C N T D K C N

  (1) Is this a new toxin? Please help him to identify this toxin.

  • Using the analysis tool on Biology Workbench , this result show that the toxin is highly homology to cardiotoxin of Naja naja atra (Chinese cobra). It may be a new toxin.

  (2) Can you find proteins that share sequence homology with this toxin? Show them in multiple alignment form.

  (3) Please predict its secondary structure.

  (4) Please show its charge distribution.

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