(a)accession number : P40692

(b)entry name : MLH1_HUMAN

(c) release date of last modification : Release 40, October 2001

number of amino acids : 756 AA

molecular weight : 84600.9 Da

theoretical pI : 5.51

Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 83
(b)
Here

Assignment 5
Sequence analysis of MLH1 protein.

 

1. Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database. Give its (a) accession number, (b) entry name and (c) release date of last modification.

Ans:

 

 

 

2. Give its number of amino acids, molecular weight and theoretical pI.

Ans:

 

 

 

3. Calculate the total number of negatively charged residues and positively charged residues.

Ans:

 

4. Calculate its hydrophobicity (Kyte & Doolittle scale, window size 11, Relative weight 60%).

Ans: Here

5. Performe the trypsin (higher specificity) cleavage of the protein. (a) How many peptides will you get after cleavage? (b) Give the list of peptides with a mass bigger than 1000 dalton

Ans: (a) 64 (Here)

 

 

 

[Answer]