(a)accession number : P40692
(b)entry name : MLH1_HUMAN
(c) release date of last modification : Release 40, October 2001
number of amino acids : 756 AA
molecular weight : 84600.9 Da
theoretical pI : 5.51
Assignment
5
Sequence
analysis of MLH1 protein.
1. Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database. Give its (a) accession number, (b) entry name and (c) release date of last modification.
Ans:
2. Give its number of amino acids, molecular weight and theoretical pI.
Ans:
3. Calculate the total number of negatively charged residues and positively charged residues.
Ans:
4. Calculate its hydrophobicity (Kyte & Doolittle
scale, window size 11, Relative weight 60%).
Ans: Here
5. Performe the trypsin (higher specificity) cleavage of the protein. (a) How many peptides will you get after cleavage? (b) Give the list of peptides with a mass bigger than 1000 dalton
Ans: (a) 64 (Here)