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PeptideMass [references] cleaves a protein sequence from the SWISS-PROT and/or TrEMBL databases or a user-entered protein sequence with a chosen enzyme, and computes the masses of the generated peptides. The tool also returns theoretical isoelectric point and mass values for the protein of interest. If desired, PeptideMass can return the mass of peptides known to carry posttranslational modifications, and can highlight peptides whose masses may be affected by database conflicts, isoforms or splicing variants.

Instructions are available.

Enter a SWISS-PROT protein identifier, ID (e.g. ALBU_HUMAN), or accession number, AC (e.g. P04406), or an amino acid sequence (e.g. 'SELVEGVIV'; you may specify post-translational modifications, but PLEASE read this document first!):

the fields. the cleavage of the protein.

The peptide masses are
with cysteines treated with:
with acrylamide adducts
with methionines oxidized
[M+H]+ or [M] or [M-H]- .
average or monoisotopic.

Select an enzyme :

Allow for missed cleavages.
Display the peptides with a mass bigger than Dalton

sorted by peptide masses or in chronological order in the protein.

For SWISS-PROT/TrEMBL entries only:
For each peptide display
all known post-translational modifications,
all database conflicts,
all variants (protein isoforms),
all varsplices (mRNA splicing variants).

Last modified 24/Jul/2001 by CHH
ExPASy Home page Site Map Search ExPASy Contact us SWISS-PROT Proteomics tools