Construction of pCEP4-hPLK

pBlue KS+-hPLK WT, K82M, T210D, ¡µKWVD cut by Not I/Xho I

                                           ¡õ
The hPLK insert are purified by electroelution
                                           ¡õ
                                           ¡õ¡ö pCE4(-EBNA) cut by Not I  /Xho I, 
                                           ¡õ        treates by CIP
                                           ¡õ
pCEP4(-EBNA)-hPLK WT, K82M, T210D, and  ¡µKWVD 

¡´Not I/Xho I digestion should produce the ~6kb vector and ~2kb hPLK


                            





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