Construction of pCEP4-hPLK
pBlue KS+-hPLK WT, K82M, T210D, ¡µKWVD cut by Not I/Xho I
¡õ The hPLK insert are purified by electroelution ¡õ ¡õ¡ö pCE4(-EBNA) cut by Not I /Xho I, ¡õ treates by CIP ¡õ pCEP4(-EBNA)-hPLK WT, K82M, T210D, and ¡µKWVD ¡´Not I/Xho I digestion should produce the ~6kb vector and ~2kb hPLK![]()
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