Margaret Dah-Tsyr Chang

    Department of Life Science
    National Tsing Hua University
    Hsinchu, Taiwan 30043, R. O. C.
    Tel:  (03)5742767
    Fax: (03)5715934
    E-mail:  lscmdt@life.nthu.edu.tw

Education:
Ph. D., Chemistry, April 1993.
The Johns Hopkins University, Baltimore, Maryland, U. S. A.
Dissertation Advisor:  Prof. Craig A. Townsend.
Dissertation Title:  "Clavaminate Synthase:  A Molecular Biological View."

M. A., Chemistry, November 1991.
The Johns Hopkins University, Baltimore, Maryland, U. S. A.

B. S., Chemistry, June 1987.
National Taiwan University, Taipei, Taiwan, R. O. C.

Experience:
1995-present Assistant Professor.  Department of Life Science, National Tsing Hua

University, Hsinchu, Taiwan, R. O. C.
Research Focus: 1.  Transactivation and regulation of mammalian stress genes.
2.  Expression and purification of human eosinophil RNases.
3.  Secretory expression of glucoamylase in yeast.
Teaching Focus: 1.  Molecular Biology.
2.  Laboratory of Molecular Biology.
3.  Bioorganic Mechanisms.
4.  Practice in Teaching Laboratory of Life Science.
1993-1995 Instructor.  Department of Life Science, National Tsing Hua University,

Hsinchu, Taiwan, R. O. C.
Research Focus: Molecular cloning and expression of vimentin.
Teaching Focus: 1.  Molecular Biology.
2.  Laboratory of Molecular Biology.
3.  Bioorganic Mechanisms.
4.  Practice in Teaching Laboratory of Life Science.
1988-1993  Research Assistant.  Department of Chemistry, The Johns
Hopkins University, Baltimore, Maryland, U. S. A.
Research Focus: Cloning and Expression of Clavaminate Synthase.
Teaching Assistant.  Department of Chemistry, The Johns Hopkins

University, Baltimore, Maryland, U. S. A.
Teaching   Laboratory of General Chemistry.
1987-1988 Teaching Assistant.  Department of Chemistry, National Taiwan

University, Taipei, Taiwan, R. O. C.
Teaching   1. Organic Chemistry.
2.  Laboratory of Organic Chemistry.

Expertise:
Molecular Biology, Genetic Engineering, Bioorganic Chemistry.

Professional Affiliations:
American Chemical Society, Biotechnology Division.
Chinese Chemical Society.
The Society for Biochemistry and Molecular Biology, Taipei, Taiwan.
The Chinese Society of Cell and Molecular Biology.
Chinese Association of Gifted Education.
Publications:

A.  Research related to PhD Study
(1).  Busby, R. W., Chang, M. D.-T., Busby, R. C., Wimp, J., Townsend,
C. A.   (1995)  Expression and purification of two isozymes of
clavaminate synthase and  initial characterization of the iron binding
site.  J. Biol. Chem. 270, 4262-4269.

(2)  Marsh, E. N., Chang, M. D.-T., Townsend, C. A. (1992)  Two isozymes
of  clavaminate synthase central to clavulanic acid formation胉loning
and sequencing of  both genes from Streptomyces clavuligerus."
Biochemistry  31, 12648-12657.

(3)  Chang, M. D.-T.*  "Clavaminate Synthase:  A Molecular Biological
View." (1992)
 PhD thesis.  Department of Chemistry, The Johns Hopkins University,
Baltimore,  Maryland, U. S. A.


B.  Research established at Department of Life Science, National Tsing
Hua  University

Journal Articles:
(1).  Chen, K.-D., Hung, J.-J., Huang, H.-L., Chang, M. D.-T., Lai,
Y.-K.  (1997)   Rapid induction of grp78 gene by cooperative actions of
okadaic acid and heat-chock in  9L rat brain tumor cells:  involvement
of a cAMP responsive element-like promoter  sequence and a protein
kinase A signaling pathway.  Eur. J. Biochem. 248, 120-129.

(2).  Chen, K.-D., Chen, L.-Y., Huang, H.-L., Lieu, C.-H., Chang, Y.-N.,
Chang, M.  D.-T., Lai, Y.-K.  (1998)  Involvement of p38
mitogen-activated protein kinase  signaling pathway in the rapid
induction of the 78 kDa glucose-regulated protein in 9L  rat brain tumor
cells.  J. Biol. Chem. 273, 749-755.

(3).  Hung, J.-J., Cheng, T.-J., Chang, M. D.-T.*, Chen, K.-D., Huang,
H.-L., Lai, Y.- K.  (1998)  Involvement of heat-chock and basal
transcription factors in differential  induction of the 70 kDa
heat-shock protein and its cognate by cadmium chloride in 9L  rat brain
tumor cells.  J. Cell. Biochem. 71, 21-35.

(4).  Hung, J.-J., Cheng, T.-J., Lai, Y.-K., Chang, M. D.-T.*  (1998)
Differential  activation of p38 mitogen-activated protein kinase and
extracellular-regulated protein  kinases confers the cadmium-induced
HSP70 expression in 9L rat brain tumor cells.  J.  Biol. Chem. 273,
31924-31931.

(5).  Hung, J.-J., Chang, M. D.-T.*, Cheng, T.-J., Lai, M.-T., Fey,
C.-H., Lai, Y., Lai,  Y. K.  (2000)  Roles of HSF1 activation in the
differential regulation of Hsc70 and  Hsp70 distinguished by treatment
with geldanamycin and cadmium chloride in 9L rat  brain tumor cells.
Revised.

Manuscript in preparation:
(1).  Lai, M.-T., Chang M. D.-T., Chao, J.-J., Cheng, N.-L., Lai, Y. K.
(2000)   Geldanamycin-elicited oxidative stress confers the enhanced
expression of Grp78 in 9L  rat brain tumor cells.

(2).  Liu, S.-W., Fey, C.-H., Ke, N.-J., Hsiung, B.-K., Chang, M.
D.-T.*  (2000)   Functional analysis of glucoamylase modified by
site-directed mutagenesis on the N- terminal domain of its signal
peptide.

(3).  Chang, H.-T., Fan, T.-c., Wu, C.-M. Chou, W.-Y., Yeung, L., Chang,
M. D.-T.*   (2000)  Fast cytotoxicity screening of recombinant
eosinophil cationic protein and  eosinophil-derived neurotoxin.

Conference Presentation:
(1).  張大慈  (1997)  Rosalind E. Franklin與DNA的世紀。張昭鼎紀念研討
會：女性科學
家的創意。台北市，中華民國。

(2).  Lee, K. R., Chiu, K. H., Lee, J. Y., Chang, M. D.-T.  (1998)
Mechanism for the  cytotoxicity of eosinophil cationic protein -studies
by computer modeling, proton,  phosphorus nuclear magnetic resonance and
mass spectroscopy.  Lecture, late breaking  section,  Experimental
Biology '98, American Association of Immunologists, San  Francisco, U.
S. A.

(3).  張大慈  (1998)  生命科學實驗教學之傳承。大學生命科學相關系所參與高
中生物
教學研習會。新竹市，臺灣，中華民國。

(4)  Fan, T.-c., Chang, H.-T., Tsai, P.-C., Wong, Y.-L, Lee, K.-R.,
Chang, M. D.-T.*   (1998) Cloning, expression, purification and
characterization of human eosinophil  cationic protein.  Poster, 1998
fall camp, The Society for Biochemistry and Molecular  Biology, Taipei,
Taiwan, R. O. C.

(5).  Hung, J.-J., Lai, Y., Lai, Y.-K., Chang, M. D.-T.*  (1998)
Transactivation and  regulation of mammalian stress proteins HSP70 and
HSC70.  Poster, 1998 fall camp,  The Society for Biochemistry and
Molecular Biology, Taipei, Taiwan, R. O. C.

(6).  張大慈*、馮蕙卿、吳奕瑩、葉雅菁、賴秀春  (1999) 大學與高中生命科學
實驗教
學之創新合作。1999年資優教育研究學術研討會：資優教育的全方位發展。台
北市，中華民國。

(7).  Chang, H.-T., Chou, W.-Y., Fan, T.-c., Wu, C.-M. Yeung, L., Lo,
J., Chang, M.
 D.-T.*  (2000)  Fast cytotoxicity screening of recombinant eosinophil
cationic protein  and eosinophil-derived neurotoxin.  Poster, Eight
Symposium on Recent Advances in  Cellular and Molecular Biology,
Ken-Ting, Taiwan, R. O. C.

(8).  Liu, S.-W., Ke, N.-J., Fey, C.-H. Hsiung, B.-K., Chang, M. D.-T.*
(2000)   Functional analysis of glucoamylase modified by site-directed
mutagenesis and  deletion.  Poster, Eight Symposium on Recent Advances
in Cellular and Molecular  Biology, Ken-Ting, Taiwan, R. O. C.

(9).  Lai, Y., Lee, C.-W., Chang, W.-m., Hung, J. J., Chang, M. D.-T.*
(2000)  Induction of HSP90s by cadmium chloride in 9L rat brain tumor
cells.  Poster, Eight  Symposium on Recent Advances in Cellular and
Molecular Biology, Ken-Ting,  Taiwan, R. O. C.