Ann-Shyn Chiang
T.H.Hseu
Jya-Wei Cheng
Haimei Huang
J.L.Yang
Y.K. Lai
Thomas S. Tan
Suh-Chin Wu
Horng-Dar Wang
Tzu-Kang Sang
Chuang-Rung Chang
 
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  Y. K. Lai
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Professor
Ph.D., North Carolina State University, 1980

Biochemistry; Genetics
email: yklai@life.nthu.edu.tw
phone (886) 3-574-2751 or
(886) 3-5715131 # 3466
fax (886) 3-5717237
 
   
 

Research Interest:
The major goal of this laboratory is to elucidate the molecular mechanisms of cellular and molecular response in stressed mammalian cells. Cells in culture respond to suboptimal conditions by reprogramming a series of metabolic processes including reorganization of cytoskeletal systems, alteration of protein phosphorylation, and induction of stress (heat shock) proteins, etc.

We are particular interested in knowing the roles of various cell signaling leading to the initiation of stress response and/or apoptotic cell death. We use heat-shock as well as a number of cytotoxic compounds as stressors and the metabolic responses are monitored in cells during and recovering from the stress treatment. Currently, several related research projects are being undertaken in this laboratory. 1. Stress response in mammalian cells - Transactivation of Stress Seventies by Protein Phosphatase Inhibitors in 9L Rat Brain Tumor Cells; 2. Molecular Mechanisms of Taxol-Induced Apoptosis in Human Leukemic HL-60 cells; and 3. Molecular Mechanism of Taxol Cytotoxicity in Human Non-Small Cell Lung Cancer H460 cells.

Selected Publications:

  1. Chao CC, Sun FC, Wang CH, Lo CW, Chang YS, Chang KJ, Chang MDT, and Lai YK. (2008). Concerted actions of multiple transcription elements confer differential transactivation of HSP90 isoforms in geldanamycin-treated 9L rat gliosarcoma cells. J Cell Biochem. 104, 1286-1296.
  2. Lin FY, Lai YK, Yu HC, Chen NY, Chang CY, Lo HC, and Hsu TH (2008). Effects of Lycium barbarum extract on production and immunomodulatory activity of the extracellular polysaccharopeptides from submerged fermentation culture of Coriolus versicolor. Food Chem. 110, 446-453.
  3. Hou, ML, Huang SY, LaiYK, and Lee WC. (2008). Geldanamycin augments nitric oxide production and promotes capacitation in boar spermatozoa. Animal Reproduction Science 104, 56-68.
  4. Shu CW, Sun FC, Cho JH, Lin CC, Liu PF, Chen PY, Chang DT, Fu HW, and Lai YK. (2008). GRP78 and Raf-1 cooperatively confer resistance to endoplasmic reticulum stress-induced apoptosis. J. Cell. Physiol. 215, 627-635.
  5. Chen TC, Lai YK, Yu CK, and Juang JL (2007). Enterovirus 71 triggering of neuronal apoptosis through activation of Abl-Cdk5 signalling. Cellular Microbiology 9, 2676-2688.
  6. Lin CC, Hsu JTA, Huang KL, Tang HK, Shu CW, and Lai YK. (2007). Sf-Caspase-I-repressed stable cells: resistance to apoptosis and augmentation of recombinant protein production. Biotechnology and Applied Biochemistry 48, 11-19.
  7. Chen PY, Chang WS, Chou RH,LaiYK, Lin SC, Chi CY, and Wu CW. (2007). Two non-homologous brain diseases-related genes, SERPINII and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner. Bmc Molecular Biology 8
  8. Chang HT, Kao YL, Wu CM, Fan TC, LaiYK, Huang KL, Chang YS, Tsai JJ, and Chang MDT. (2007). Signal peptide of eosinophil cationic protein upregulates transforming growth factor-alpha expression in human cells. Journal of Cellular Biochemistry 100, 1266-1275.
  9. Chen CC, Tai DY, Ho P, Lai YK, Lin JJ, Mao SJT, Lin CC, Hsieh YC, Chiang HC, and Dai KS (2007). Clinical evaluation of the eBsensor hand-held blood glucose monitoring system. Clinica Chimica Acta 377, 170-173.
  10. Yang DG, Chung YC, LaiYK, Lai CW, Liu HJ, and Hu YC. (2007). Avian influenza virus hemagglutinin display on baculovirus envelope: Cytoplasmic domain affects virus properties and vaccine potential. Molecular Therapy 15, 989-996.
  11. HuangKS, Lo WH, Chung YC, Lai YK, Chen CY, Chou ST, and Hu YC. (2007). Combination of baculovirus-mediated gene delivery and packed-bed reactor for scalable production of adeno-associated virus. Human Gene Therapy 18, 1161-1170.
  12. Sun FC, Wei S, Li CW, Chang YS, Chao CC, and Lai YK. (2006). Localization of GRP78 to mitochondria under the unfolded protein response. Biochemical Journal. 396(1), 31-9.
  13. Lin CC, Hsu JT, Huang KL, Tang HK,and Lai YK. (2006). Stable RNA Interference in Spodoptera frugiperda Cells by a DNA Vector-based Method. Biotechnology Letters. 28(4), 271-7.
  14. Chen LC, Lai YK, WuSC, Lin CC, and Guo JH (2006). Production by Clonostachys compactiuscula of a lovastatin esterase that converts lovastatin to monacolin. J. Enzyme and Microbial Technology. 39(5), 1051-1059.
  15. Chang YS, Lo CW, Sun FC, Chang MD, andLaiYK. (2006). Differential expression of Hsp90 isoforms in geldanamycin-treated 9L cells. Biochemical and Biophysical Research Communications. 344(1), 37-44.
  16. Chen TC, Chen GW, Hsiung CA, Yang JY, Shih SR, Lai YK, and Juang JL. (2006). Combining multiplex reverse transcription-PCR and a diagnostic microarray to detect and differentiate enterovirus 71 and coxsackievirus A16. Journal of clinical microbiology. 44(6), 2212-9.
  17. Lai CW, Chan ZR, Yang DG, Lo WH, LaiYK, Chang MD, and Hu YC. (2006). Accelerated induction of apoptosis in insect cells by baculovirus-expressed SARS-CoV membrane protein. FEBS Letters. 580, 3829¡V3834.
  18. Huang KL, LaiYK, Lin CC, and Chang JM. (2006). Inhibition of hepatitis B virus production by Boehmeria nivea root extract in HepG2 2.2.15 cells. World journal of gastroenterology 12(35), 5721-5.
  19. Chang YS, Lee LC, Sun FC, Chao CC, Fu HW, and Lai YK. (2006). Involvement of calcium in the differential induction of heat shock protein 70 by heat shock protein 90 inhibitors, geldanamycin and radicicol, in human non-small cell lung cancer H460 cells. J Cell Biochem. 97(1), 156-65.
  20. Lai CW, Chung YC, Lai YK, Chang MD, and Hu YC (2005). Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study. Biotechnol Lett. 27(13), 883-91.
  21. Shu CW, Cheng NL, Chang WM, Tseng TL, and Lai YK (2005) Transactivation of hsp70-1/2 in Geldanamycin-Treated Human Non-Small Cell Lung Cancer H460 Cells: Involvement of Intracellular Calcium and Protein Kinase C. J. Cell. Biochem. 94(6), 1199-209.
  22. Juang JL, Chen TC, Jiang SS, Hsiung CA, Chen WC, Chen G.W, Lin SM, Lin JH, Chiu SC, and Lai YK, (2004).Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.Lab Invest. 84, 1085-1091.
  23. Lai, M.-T., K.-L. Huang, W.-M. Chang and Y.-K. Lai, Geldanamycin Induction of grp78 Requires Activation of Reactive Oxygen Species via ER Stress Responsive Elements in 9L Rat Brain Tumor Cells. Cell. Signal. 15, 585-595 (2003)
  24. Cheng, T.-J., Y.-F. Tseng, W.-M. Chang, M. D.-T. Chang and Y.-K. Lai, Retaining of the Assembly Capability of Vimentin Phosphorylated by Mitogen-Activated Protein Kinase-Activated Protein Kinase-2. J. Cell. Biochem. 79, 589-602 (2003)
  25. Chang, W.-M., K.-D. Chen, L.-Y. Chen, M.-T. Lai and Y.-K. Lai, Mitochondrial Calcium-Mediated Reactive Oxygen Species Are Essential for the Rapid Induction of the grp78 Gene in 9L Rat Brain Tumour Cells. Cell. Signal. 15, 57-64 (2002)
  26. Smbaiah, T., K.-Y. King, S.-C. Tsay, N.-W. Mei, S. Kakimclahi, Y.-K. Lai , C.-H. Lieu and J.-R. Hwu, Synthesis and immunofluorescence assay of a new biotinylated paclitaxel. Eur. J. Med. Chem. 37, 349-353 (2002)
  27. Cheng, T.-J., Y.-L. Lin, A.-S. Chiang and Y.-K. Lai, Association of Protein Phosphatase 2A with its Substrate Vimentin Intermediate Filaments in 9L Rat Brain Tumor Cells J. Cell. Biochem. 79, 126-138 (2000)
  28. Chu, J.-J., C.-D. Chiang, C.S. Rao, W.-M. Chang and Y.-K. Lai, Establishment and Characterization of a Paclitaxel-Resistant Human Non-Small Cell Lung Cancer Cell Line. Anticancer Res. 20, 2449-2456 (2000)
  29. Chen, L.-Y., A.-S. Chiang, J.-J. Hung, H.-I. Hung and Y.-K. Lai , Thapsigargin-Induced Grp78 Expression is Mediated by the Increase of Cytosolic Free Calcium in 9L Rat Brain Tumor Cells. J. Cell. Biochem. 78, 404-416 (2000).(IF: 3.672)
  30. Chen, K.-D., M.-T. Lai, J.-J. Hung, L.-Y. Chen and Y.-K. Lai, Activation of p38 Mitogen-Activated Protein Kinase and Mitochondrial Ca2+-Mediated Oxidative Stress Are Essential for the Enhanced Expression of grp78 Induced by the Protein Phosphatase Inhibitors Okadaic Acid and Calyculin A. J. Cell. Biochem. 76, 585-595 (2000)
  
 
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