Protein X Sequence

LKKVV ALYDY MPMNA NDLQL RKGDE YFILE ESNLP WWRAR DKNGQ EGYIP SNYVT EAE
  

1.What is Protein X

This is not a new protein because it is 100% identical with Btk 
[ Homo sapiens ],  and it also has high homology to other 
tyrosine kinase.
The name of this protein is "Bruton's agammaglobulinemia tyrosine
kinase"

  

2.Come back to its DNA sequence

From Human Bruton's agammaglobulinemia tyrosine kinase(Btk) gene,
it has 19 exons and 18 introns. Please choose the sequence which 
you want to see from the following table.
Exon 1- 19
exon 1 exon 2 exon 3 exon 4 exon 5 exon 6 exon 7 exon 8 exon 9 exon 10
exon 11 exon 12 exon 13 exon 14 exon 15 exon 16 exon 17 exon 18 exon 19
Intron 1- 18
intron 1 intron 2 intron 3 intron 4 intron 5 intron 6 intron 7 intron 8 intron 9 intron 10
intron 11 intron 12 intron 13 intron 14 intron 15 intron 16 intron 17 intron 18

  

3.Reference of this protein

REFERENCE    1  (residues 1 to 657)
  AUTHORS    Hagemann,T.L., Chen,Y., Rosen,F.S. and Kwan,S.P.
  TITLE      Genomic organization of the Btk gene and exon scanning for
             mutations in patients with X-linked agammaglobulinemia
  JOURNAL    Hum. Mol. Genet. 3 (10), 1743-1749 (1994)
  MEDLINE    95152493

REFERENCE    2  (residues 1 to 657)
  AUTHORS    Kwan,S.
  TITLE      Direct Submission
  JOURNAL    Submitted (12-AUG-1994) Sau-Ping Kwan,
             Immunology/Microbiology, Rush Medical   School, 1750 
             W. Harrison St., Chicago, IL 60612, USA

REFERENCE    3  (bases 1 to 502)
  AUTHORS    Sideras,P., Muller,S., Shiels,H., Jin,H., Khan,W.N.,
             Nilsson,L., Parkinson,E., Thomas,J.D., Branden,L.,
             Larsson,I., Paul,W.E., Rosen,F.S., Alt,F.W., Vetrie,D.,
             Smith,C.I.E. and Xanthopoulos,K.G.
  TITLE      Genomic organization of mouse and human Bruton's
             agammaglobulinemia tyrosine kinase (Btk) loci
  JOURNAL    J. Immunol. 153 (12), 5607-5617 (1994)
  MEDLINE    95081608

  

4.What is Btk

Btk is a cytoplasmic protein tyrosine kinase (PTK) that has been 
directly implicated in the pathogenesis of X-linked agammaglobulinaemia
(XLA) in humans and X-linked immunodeficiency (Xid) in mice. And this
cytoplasmic protein tyrosine kinase is of the Src family designated 
Btk (Bruton's tyrosine kinase).

Btk amino acid sequence:

     MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCV
     ETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQL
     KNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTP
     EEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARD
     KNGQEGYIPSNYVTEAEDSIEMWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAK
     STGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPS
     TAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMM
     NLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLES
     KQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKS
     DIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERP
     TFKILLSNILDVMDEES


E-mail:­J©y§Õ u821633@life.nthu.edu.tw