Q: A protein with extinction coefficient E^1% = 16 yields an absorbance of 0.73 when measured in a 0.5-cm cell. Calculate the weight concentration.

Solution

The absorbance in E^1% can be written as,

A = E^1% * b * c

Where A = 0.73 in a 0.5-cm cell, and = 16. Then, the weight concentration is

c = A / (E^1% * b) = 0.73 / (16*0.5) = 0.091 g/100ml