C13 spectrum
of 100mM SDS:
(A) pure SDS
(B) 1/80 12-DXSA(12-doxyl-stearic acid)
(C) 1/80 5-DXSA(5-doxyl-stearic acid)
This results indicate that the spin-label moiety of
12-DXSA is near the center of SDS micelle while 5-DXSA is closer to the
micellar surface. |
Interaction between spin-label
and the peptides can be determined by NMR cross peaks. |
 |