Mutants' Stabilities
		Ala16 and Leu49 are two of the most important residues constituting the main hydrophobic core of WT-CI2.A. GdnHCl denatured fluorescence curve. Left: LA49(I)+WT(II) Right: AG16(I)+WT(II) (Ex 280nm, Em 356nm) B.By stopped-flow fluorescence: (Left hand side) Top: LA49(I)+WT(II) and AG16(I)+WT(II) Meddle: "Slower phase" of LA49(I)+WT(II) Bottom: "Slower phase" of AG16(I)+WT(II) The result showed that LA49(I)+WT(II) and AG16(I)+WT(II) were described by two-state transition, and they did not populate a stable intermediate. Hydrophobic core effect refolding!