homework3

  • due on 5/5

1. Please find the genes for the protein that you showed the structures in homework 2.

1 ctcatcatca gcaaccaatt cgcaccgatc gatcgatcga tccagcaact agatcaacag

61 tactagctag gaagcatggc ccgtgcacag ttggtgttgg tcgccctggt ggcagctctg

121 ctcctcgccg ccggccacgc cgccgtggcc atcacctgcg cccaggtcaa ctccgccgtc

181 gggccctgcc tgacctacgc ccgcggcggc gtcgggccct cggccgcctg ctgcagcggc

241 gtgaggagcc tcaaggcggc agcaagcacg accgcagacc ggcgcaccgc ctgcaactgc

301 ctcaagaacg cggcccgcgg catcaagggg ctcaacgccg gcaacgccgc cagcatcccc

361 tccaagtgcg gcgtcagcgt cccctacacc atcagcgctt ccatcgactg ctccagggtg

421 agctgagcca tcgatcagag acggatcata tatatacagc agcgcgccgg ttgccaccta

481 gtagattctg cctgggtcga tgtgtggagc c

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2. Which organism do these genes come from? How many sequences of DNA and Protein have been known for this organism?

ORGANISM: Oryza sativa(Rice)
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69340 nucleotides sequences and 2255 protein sequence have been know for Oryza sativa(rice).

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3. How many BLAST hits you can get if you use the gene you found for this protein as the query sequence to search against non-redundant GenBank + EMBL + DDBJ + PDB sequence database? Please also show 10 sequences that produce significant alignments ( you just need to show their names and ID numbers ).

we can get 95 BLAST hits.

Name

ID number

phospholipid transfer protein homolog - rice EPRZ
NONSPECIFIC LIPID-TRANSFER PROTEIN 1 PRECURSOR (LTP 1) (PAPI) P23096
lipid transfer protein LPT IV AAB70541
phospholipid transfer protein AAB06443
NONSPECIFIC LIPID-TRANSFER PROTEIN 2 PRECURSOR (LTP 2) Q43194
NONSPECIFIC LIPID-TRANSFER PROTEIN 2 PRECURSOR (LTP 2) Q42978
lipid transfer protein LPT II AAB70539
NONSPECIFIC LIPID-TRANSFER PROTEIN 3 PRECURSOR (LTP 3) Q42999
NONSPECIFIC LIPID-TRANSFER PROTEIN 1 PRECURSOR (LTP 1) Q43193
lipid transfer protein - sorghum (fragment) S33460