ProtParam tool
User provided sequence:
IRPPYLALQC PPLSPALPRR VPPRPCPAGM QRSPPGYGAQ DDPPSRRDCA WAPGIGAAAE
ARGLPVTNVS PTSPASPSSL PRSPPRSPES GRYGFGRGER QTADELRIRR PMNAFMVWAK
DERKRLAQQN PDLHNAVLSK MLGKAWKELN TAEKRPFVEE AERLRVQHLR DHPNYKYRPR
RKKQERKVRR LEPGLLLPGL VQPSAPPEAF AAASGSARSF RELPTLGAEF DGLGLPTPER
SPLDGLEPGE ASFFPPPLAP EDCALRAFRA PYAPELARDP SFCYGAPLGE ALRTAPPAAP
LAGLYYGTLX XPGPXXNPLS PPPESPSLEG TEQLEPTADL WADVDLTEFD QYLNCSRTRP
DATTLPYHVX LAKLGPRAMS CPEESSLISA LSDASSAVYY SACISG
References and documentation are available.
Number of amino acids: 406
Molecular weight: 44178.8
Theoretical pI: 8.58
Amino acid composition:
Ala (A) 48 11.8%
Arg (R) 38 9.4%
Asn (N) 8 2.0%
Asp (D) 17 4.2%
Cys (C) 8 2.0%
Gln (Q) 11 2.7%
Glu (E) 28 6.9%
Gly (G) 27 6.7%
His (H) 4 1.0%
Ile (I) 5 1.2%
Leu (L) 44 10.8%
Lys (K) 11 2.7%
Met (M) 5 1.2%
Phe (F) 11 2.7%
Pro (P) 61 15.0%
Ser (S) 32 7.9%
Thr (T) 14 3.4%
Trp (W) 4 1.0%
Tyr (Y) 13 3.2%
Val (V) 12 3.0%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 5 1.2%
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 49
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 41030 41108 40605 39880 38480
Abs 0.1% (=1 g/l) 0.929 0.930 0.919 0.903 0.871
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 40450 40600 40125 39400 38000
Abs 0.1% (=1 g/l) 0.916 0.919 0.908 0.892 0.860
Estimated half-life:
The N-terminal of the sequence considered is I (Ile).
The estimated half-life is: 20 hour (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hour (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 72.51
This classifies the protein as unstable.
Aliphatic index: 67.46
Grand average of hydropathicity (GRAVY): -0.562