Homework 3

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1.Please find the genes for the protein that you showed the structures in homework 2.

1 ctcatcatca gcaaccaatt cgcaccgatc gatcgatcga tccagcaact agatcaacag

61 tactagctag gaagcatggc ccgtgcacag ttggtgttgg tcgccctggt ggcagctctg

121 ctcctcgccg ccggccacgc cgccgtggcc atcacctgcg cccaggtcaa ctccgccgtc

181 gggccctgcc tgacctacgc ccgcggcggc gtcgggccct cggccgcctg ctgcagcggc

241 gtgaggagcc tcaaggcggc agcaagcacg accgcagacc ggcgcaccgc ctgcaactgc

301 ctcaagaacg cggcccgcgg catcaagggg ctcaacgccg gcaacgccgc cagcatcccc

361 tccaagtgcg gcgtcagcgt cccctacacc atcagcgctt ccatcgactg ctccagggtg

421 agctgagcca tcgatcagag acggatcata tatatacagc agcgcgccgg ttgccaccta

481 gtagattctg cctgggtcga tgtgtggagc c

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2.Which organism do these genes come from?

Oryza sativa

How many sequences of DNA and Protein have been known for this organism?

72192 nucleotide and 2257 protein sequences have been known

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3.How many BLAST hits you can get if you use the gene you found for this protein as the query sequence to search against non-redundant GenBank + EMBL + DDBJ + PDB sequence database?

95 BLAST hits

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Please also show 10 sequences that produce significant alignments ( you just need to show their names and ID numbers ).

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ID number

Name
pir||EPRZ phospholipid transfer protein homolog - rice >gi|4139
gi|2407277 (AF017361) lipid transfer protein LPT IV [Oryza sativa]
sp|P23096| NLT1_ORYSA NONSPECIFIC LIPID-TRANSFER PROTEIN 1 PRECU
gi|1498597 (U66105) phospholipid transfer protein [Zea mays]
sp|Q43194|NLT2_SORVU NONSPECIFIC LIPID-TRANSFER PROTEIN 2 PRECU
sp|Q42978|NLT2_ORYSA NONSPECIFIC LIPID-TRANSFER PROTEIN 2 PRECU
gi|2407273 (AF017359) lipid transfer protein LPT II [Oryza sativa]
gi|2407275 (AF017360) lipid transfer protein LPT III [Oryza sat]
gi|2407271 (AF017358) lipid transfer protein [Oryza sativa]
sp|Q43193|NLT1_SORVU NONSPECIFIC LIPID-TRANSFER PROTEIN 1 PRECU