Jun-Hung Cho                                                                                                    Room N301, Institute of Biomedical Sciences,

Academia Sinica, No. 128, Academy Rd., Sec. 2, Taipei City, Taiwan 11529, R.O.C.  

home: 4F., No.8, Lane 25, Dapu St., Jhongjheng District, Taipei City 100, Taiwan (R.O.C.)

Phone: 886-2-2789-9127

Fax: 886-2-2785-8594

E-mail: table@ibms.sinica.edu.tw

                                                                                                                                                                                                                                                              


Research Assistant
Institute of Biomedical Sciences                                 
Academia Sinica			    
Sex: Male
Birth: 9/9/1975

OBJECTIVE                        2005 Fall Ph.D. student

                        To enroll as a 2005 fall Ph.D. student with research interests in cell biology and Biomedical Sciences. 

　
EDUCATION
M.S.	9/1998~6/2000 	National Tsing Hua University	Hsinchu, Taiwan
             Institute of life science, Advisors: Professor  Yiu-Kay Lai
	GPA: 3.66/4.0
	Master's Thesis: Involvement of Protein Kinase C in the Geldanamycin-Induced Transactivation of Hsp70-1 
in Human Non-Small Cell Lung Cancer H460 Cells Jun-Hung Cho, Whei-meih Chang, Nai-Lin Cheng, Yiu-Kay Lai§ submitted to the journal cellular biochemistry.
                             
B.S. 	9/1994~6/1998	National Sun Yat-sen University (NSYSU) University	Kaohsiung, Taiwan
              Department of Marine Resources
	Overall GPA: 2.97/4.0
	Last 60 Credits: 3.5/4.0

My thesis paper

#1Involvement of Protein Kinase C in the Geldanamycin-Induced Transactivation of Hsp70-1 in Human Non-Small Cell Lung Cancer H460 Cells.

#2conference poster in 2000 australia

CONFERENCE PAPER and published papers

1. Activation of p38 mitogen-activated protein kinase and mitochondrial Ca(2+)-mediated oxidative stress are essential for the enhanced expression of grp78 induced by the protein phosphatase inhibitors okadaic acid and calyculin A.

 Chen KD, Lai MT, Cho JH, Chen LY, Lai YK. J Cell Biochem. 2000 Jan;76(4):585-95.

2.Involvement of Protein Kinase C in the Geldanamycin-Induced Transactivation of Hsp70-1 in Human Non-Small Cell Lung Cancer H460 Cells. Jun-Hung Cho, Whei-meih Chang, Nai-Lin Cheng, Yiu-Kay Lai§ submitted to the journal cellular biochemistry.

3.INCREASE OF CYTOSOLIC CALCIUM IS INVOLVED IN THE THPASGARGIN-INDUCED grp78 EXPRESSION.

Liuh-Yow Chen, Jun-Hung Cho, Ming-tsong Lai, Yui-Kay Lai

conference poster in Hong Kong Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan 30013, Republic of China  

4.Geldanamycin Induces Transactivation of Hsp70a in Human Non-Small Cell Lung Cancer H460 Cells through Calcium Sensitive and Non-Sensitive Pathway . Nai-Lin Cheng, Jun-Hung Cho, Yiu-Kay Lai§in press   From the Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan 30013, Republic of China      Powerpoint File

    PROFESSIONAL EXPERIENCE

　

04/2001~                                    Academia Sinica                        Taipei, Taiwan

           Institute of Biomedical Sciences

Research Assistant  in Dr. Tang K. Tang

l      Participated in the project “novel protein play important role in genomic instability”.

　

07/2000~04/2001                       Academia Sinica                        Taipei, Taiwan

           Institute of Biomedical Sciences

Research Assistant  in Dr. Joan ne Jeou-Yuan Chen

l      Participated in the project “screen potential tumor marker in gastric cancer”.

07/1998~06/2000                        National Tsing Hua University Hsinchu, Taiwan

                    Department of Life Sciences

              

l       stress protein  regulation in cancer cells.

COMPUTERS

Softwares: Microsoft Office, vector NT1, photoshop,visio, familiar with bioinformatics website to solve the biology problems.

PROFESSIONAL SKILLS

Biotechniques:

Molecular Cloning, northern blot, southern blot,Western Blot, RT-PCR, Site-Direct Mutagenesis, Inverse PCR, Semi-Quantitative RT-PCR, DNA Recombination in Bacterial Cells, Prokaryotic Expression System and Protein Purification.(Gst and pet system)

cell biology

flow cytometry , cell cycle stage study, kinase assay, transient transfection. siRNA treatment ( oligo and vector based). immuofloresence observed cell in different cell cycle stages. stable clone selection.

Cytology:

Cell Culture, MTT Assay, Immunocytochemistry, β-Galatosidase Staining, Luciferase Reporter Assay,  Confocal Microscope.

Gene Transfer:

Electroporation,  Calcium Phosphate Precipitation, loposome, lipofectamine2000

biochemistry PAGE in protein, EMSA (electrophoretic mobility shift assay), phosphoamino acid analysis, peptide mapping

EXTRACURRICULAR ACTIVITIES

09/96~06/98                        Minister                        Division of Activities, Student  Association                                                NSYSU

09/94~07/98                       Member                        Varsity of Basketball                                                                                    NSYSU

09/96~06/98                       Team leader                   Varsity of Basket of department

09/00~06/04                        Member                        Varsity of Basketball  in academic sinica                         

                                                                                                                                                                  計數器