Double Staining. Rat cortical neuron were fixed and permeabilized.
For 1st stain, Oregon Green conjugated Phalloidin was used to localized actin; for 2nd stain, primary antibody is anti-tubulin mouse IgG, secondary antibody is Texas Red
conjugated anti mouse IgG. Here shows a typical Actin-Tubulin distrubution profile of Growth cone(1000*) |
Double Staining. Rat cortical neuron were fixed and permeabilized.
For 1st stain, Oregon Green conjugated Phalloidin was used to localized actin; for 2nd stain, primary antibody is anti-tubulin mouse IgG, secondary antibody is Texas Red
conjugated anti mouse IgG.(400*) |