Homework 7-4
CHARGE DISTRIBUTIONAL ANALYSIS
Charge Distribution Assay : 1 0+00+00000 0+00000+00
00+0000000 +000++000- 000+00000+ 000000-+00
A. CHARGE CLUSTERS. Positive charge clusters
(cmin = 13/30 or 18/45 or 22/60): none Negative charge clusters: not
evaluated (frequency of - < 5%, too low) Mixed charge clusters
(cmin = 15/30 or 20/45 or 25/60): none
B. HIGH SCORING (UN)CHARGED SEGMENTS. High
scoring positive charge segments: score= 2.00 frequency= 0.183 ( KR )
score= 0.00 frequency= 0.000 ( BZX ) score= -1.00 frequency= 0.783 (
LAGSVTIPNFQYHMCW ) score= -2.00 frequency= 0.033 ( ED ) Expected
score/letter: -0.483 - now scoring for positive charge segments;
Average information/letter: 0.430 Minimal length of displayed
segments set to: 20 M_0.01= 13.07 (cv= 7.77, lambda= 0.52686, k=
0.16371, x= 5.30; 90% confidence interval for segment length: 23 +-
25) M_0.05= 9.97 (x= 2.20) # of segments (>=20 residues) exceeding
M_0.05: none High scoring negative charge segments: score= 2.00
frequency= 0.033 ( ED ) score= 0.00 frequency= 0.000 ( BZX ) score=
-1.00 frequency= 0.783 ( LAGSVTIPNFQYHMCW ) score= -2.00 frequency=
0.183 ( KR ) Expected score/letter: -1.083 - now scoring for negative
charge segments; Average information/letter: 3.490 Minimal length of
displayed segments set to: 20 M_0.01= 4.94 (cv= 2.54, lambda=
1.61122, k= 0.47671, x= 2.40; 90% confidence interval for segment
length: 3 +- 3) M_0.05= 3.92 (x= 1.38) # of segments (>=20
residues) exceeding M_0.05: none High scoring mixed charge segments:
score= 1.00 frequency= 0.217 ( KEDR ) score= 0.00 frequency= 0.000 (
BZX ) score= -1.00 frequency= 0.783 ( LAGSVTIPNFQYHMCW ) Expected
score/letter: -0.567 - now scoring for mixed charge segments; Average
information/letter: 1.051 Minimal length of displayed segments set
to: 20 M_0.01= 6.07 (cv= 3.19, lambda= 1.28520, k= 0.40993, x= 2.89;
90% confidence interval for segment length: 11 +- 9) M_0.05= 4.80 (x=
1.62) # of segments (>=20 residues) exceeding M_0.05: none High
scoring uncharged segments: score= 1.00 frequency= 0.783 (
LAGSVTIPNFQYHMCW ) score= 0.00 frequency= 0.000 ( BZX ) score= -8.00
frequency= 0.217 ( KEDR ) Expected score/letter: -0.950 - now scoring
for uncharged segments; Average information/letter: 0.173 Minimal
length of displayed segments set to: 20 M_0.01= 32.53 (cv= 20.56,
lambda= 0.19916, k= 0.10900, x= 11.97; 90% confidence interval for
segment length: 54 +- 44) M_0.05= 24.34 (x= 3.79) # of segments
(>=20 residues) exceeding M_0.05: none
C. CHARGE RUNS AND PATTERNS. pattern (+)| (-)|
(*)| (0)| (+0)| (-0)| (*0)|(+00)|(-00)|(*00)| lmin0 5 | 3 | 6 | 29 |
10 | 6 | 10 | 11 | 6 | 12 | lmin1 6 | 4 | 7 | 36 | 12 | 7 | 12 | 14 |
8 | 14 | lmin2 7 | 4 | 8 | 39 | 13 | 8 | 14 | 15 | 9 | 16 | There are
no charge runs or patterns exceeding the given minimal lengths. Run
count statistics: + runs >= 3: 0 - runs >= 3: 0 * runs >= 4:
0 0 runs >= 20: 0