Input Sequence

P04406 (334 aa) 

GKVKVGVNGF GRIGRLVTRA AFNSGKVDIV AINDPFIDLN YMVYMFQYDS
THGKFHGTVK AENGKLVING NPITIFQERD PSKIKWGDAG AEYVVESTGV
FTTMEKAGAH LQGGAKRVII SAPSADAPMF VMGVNHEKYD NSLKIISNAS
CTTNCLAPLA KVIHDNFGIV EGLMTTVHAI TATQKTVDGP SGKLWRDGRG
ALQNIIPAST GAAKAVGKVI PELNGKLTGM AFRVPTANVS VVDLTCRLEK
PAKYDDIKKV VKQASEGPLK GILGYTEHQV VSSDFNSDTH SSTFDAGAGI
ALNDHFVKLI SWYDNEFGYS NRVVDLMAHM ASKE

Results of Subprograms

*** Warning: 1st aa is not methyonine

PSG:  a new signal peptide prediction method
      N-region:  length 4;  pos.chg 2;  neg.chg 0
      H-region:  length 7;  peak value  -2.71
      PSG score:  -7.11

GvH:  von Heijne's method for signal seq. recognition
      GvH score (threshold: -2.1): -12.15
      possible cleavage site: between 25 and 26

>>> Seems to have no N-terminal signal peptide

ALOM: Klein et al's method for TM region allocation
      Init position for calculation: 1
      Tentative number of TMS(s) for the threshold  0.5:   0
      number of TMS(s) .. fixed
      PERIPHERAL  Likelihood =  1.06 (at 118)
      ALOM score:   1.06  (number of TMSs: 0)

MITDISC: discrimination of mitochondrial targeting seq
      R content:       3       Hyd Moment(75):  5.09
      Hyd Moment(95):  3.72    G content:       6
      D/E content:     1       S/T content:     2
      Score: -4.93

Gavel: prediction of cleavage sites for mitochondrial preseq
      R-2 motif at 29  TRA|AF

NUCDISC: discrimination of nuclear localization signals
      pat4: none
      pat7: none
      bipartite: none
      content of basic residues:  10.8%
      NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals: 
      KKXX-like motif in the C-terminus: MASK

SKL: peroxisomal targeting signal in the C-terminus: none

SKL2: 2nd peroxisomal targeting signal:  none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
      type 1: none
      type 2: none

NMYR: N-myristoylation pattern : none

Prenylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: none

Dileucine motif in the tail: none

checking 63 PROSITE DNA binding motifs:  none

checking 71 PROSITE ribosomal protein motifs:  none

checking 33 PROSITE prokaryotic DNA binding motifs:  none

Results of the k-NN Prediction

k = 25

         44.0 %: cytoplasmic
         20.0 %: nuclear
         16.0 %: cytoskeletal
          8.0 %: mitochondrial
          4.0 %: extracellular, including cell wall
          4.0 %: plasma membrane
          4.0 %: vacuolar

>> prediction for P04406 is cyt