Input Sequence

QUERY (1332 aa) 

MRRKTVTKGT AIVNSRSARR AATTICNFFL IALVALSSTT LADESIDTRE
NADLTLKCRF NDKYEANDFS FFWTRWTANP AQFDNVAIGE VQLSSGYRLD
FQPERGIYDL QIKNVSYNRD NGRFECRIKA KGTGADVHQE FHNLTVLTPP
HPPVISPGNI AVATEDKPME LTCSSIGGSP DPTITWYREG SNTPLPATVL
KGGTKDQPTN ATLSIIPRRE DDGAKYKCVV RNRAMNEGKR LEATATLNVN
YYPRVEVGPE NPLRVERDRT AKLECNVDAK PKVPNVRWNR NGRFISSSLV
HTIHRVSVQD AGKYTCIADN GLGKTGEQEL ILDILYPPMV VIESKTREAE
EGDTVTIRCN VTANPAPVTI EWLKENSPDF RYNGDVLTLT SVRADHAGNY
ICRAVNIMQS QGMERSERVG NSTVALLVRH RPGQAYITPN KPVVHVGNGV
TLTCSANPPG WAVPQYRWFR DMDGEFSSTQ KILAQGPQYS IPKAHLGNEG
KYHCHAVNEL GIGMMATIVL EIHQPPQFLA KLQQHMTRRV ADTDYTVTCS
AKGKPAPSVK WLKDAVEILP EENLYEVQTN PDQGLNGMVT VQSQLKFRGK
ARPNGNALVP GDRGLYTCLY QNEVNSANSS MQLRIEHEPI VLHQYNKVAF
DIRETAEVVC KVQAYPKPEF QWQFGNNPSP LTMSSDGHYE ISTTTDNNDI
YTSVLKINSL THSDYGEYTC RVANTLDTIR APIRLQQKGP PEKPTNLRAT
EVGHNYVSLS WDPGFDGGLS KTKFFVSYRR VAMPREEQLI PDCATLANSN
SAWVEVDCQR DIPCKVTALE QHQSYAFKVK ALNPKSDSPY SSEIMVTTKV
SRIPPPLQVT YEPNTRTLGI DVGATCLNLV AVVESMVNAD SPMAAWEVVT
TMDNLQLSGN GPTHKEKIID RIIGARRVGG GRALGHTISE DEDDNGLNSL
ALEDENSPTV RVKLCLRTNP EHCGDYTDAE IGRPYIAEAN ALATPTLIAI
IVSCVVFALF AGLILMFCRC KRNQSKKSAA AKDYEMDSVR PSIVAAQQNQ
APPPYYPASG LDNKALEHSM DLALSMEDQK TALYATQNGY SYHPESGVVG
VGMAGGVVGV GVGGSVVSGM GGGVGGIGGS GVGVNGIPGL SAHTMPGNEW
VNMGYMENNY SNSNNGGSVN SQDSLWQVKM SAAAVGNQQG MVQAPMNQYV
EQQPAYGYDP LTHGGYGAVD DYAPYPHLTA TPSQVGDEYH NLRNSQNPSR
QDYCSDPYAS VQKPKKRVDQ HLDSPYHDVS GLPNPYNMEH LEQDEVLPPQ
QHMSLSYDDS FEGEYSTTPN ARNRRVIREI IV

Results of Subprograms

PSG:  a new signal peptide prediction method
      N-region:  length 8;  pos.chg 4;  neg.chg 0
      H-region:  length 7;  peak value  -3.66
      PSG score:  -8.06

GvH:  von Heijne's method for signal seq. recognition
      GvH score (threshold: -2.1):   2.78
      possible cleavage site: between 42 and 43

>>> Seems to have no N-terminal signal peptide

ALOM: Klein et al's method for TM region allocation
      Init position for calculation: 1
      Tentative number of TMS(s) for the threshold  0.5:   4
      INTEGRAL    Likelihood = -3.88   Transmembrane   21 -  37
      INTEGRAL    Likelihood = -1.44   Transmembrane  867 - 883
      INTEGRAL    Likelihood =-12.52   Transmembrane  998 -1014
      INTEGRAL    Likelihood = -1.59   Transmembrane 1097 -1113
      PERIPHERAL  Likelihood =  1.06 (at 506)
      ALOM score: -12.52  (number of TMSs: 4)

MTOP: Prediction of membrane topology (Hartmann et al.)
      Center position for calculation: 28
      Charge difference: -7.0   C(-3.0) - N( 4.0)
      N >= C: N-terminal side will be inside

>>> membrane topology: type 3a

MITDISC: discrimination of mitochondrial targeting seq
      R content:       5       Hyd Moment(75): 16.53
      Hyd Moment(95):  5.92    G content:       1
      D/E content:     1       S/T content:    11
      Score:  3.83

Gavel: prediction of cleavage sites for mitochondrial preseq
      R-2 motif at 30  RRA|AT

NUCDISC: discrimination of nuclear localization signals
      pat4: KPKK (4) at 1263
      pat4: PKKR (4) at 1264
      pat7: PKKRVDQ (5) at 1264
      pat7: PNARNRR (3) at 1319
      bipartite: none
      content of basic residues:   9.7%
      NLS Score:  0.72

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals: 
      XXRR-like motif in the N-terminus: RRKT

none

SKL: peroxisomal targeting signal in the C-terminus: none

SKL2: 2nd peroxisomal targeting signal:  none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
      type 1: none
      type 2: none

NMYR: N-myristoylation pattern : none

Prenylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: none

Dileucine motif in the tail: none

checking 63 PROSITE DNA binding motifs:  none

checking 71 PROSITE ribosomal protein motifs:  none

checking 33 PROSITE prokaryotic DNA binding motifs:  none

NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination
      Prediction: nuclear
      Reliability: 55.5

COIL: Lupas's algorithm to detect coiled-coil regions
      total: 0 residues 

	 34.8 %: plasma membrane
	 21.7 %: endoplasmic reticulum
	 17.4 %: nuclear
	  8.7 %: mitochondrial
	  4.3 %: extracellular, including cell wall
	  4.3 %: cytoskeletal
	  4.3 %: cytoplasmic
	  4.3 %: peroxisomal

>> prediction for QUERY is pla (k=23)