蝴矹フ筿猘だ猂
10 % SDS-PAGE
Separation gel mixture |
Stacking gel mixture |
| 1.5 M Tris pH8.8 2.5 ml | 1.0 M Tris pH6.8 0.38 ml |
| ddH2O 4.0 ml | ddH2O 2.1 ml |
| 10 % SDS 0.1 ml | 10 % SDS 0.03ml |
| 30 % Acrylamide 3.3 ml | 30 % Acrylamide 0.5 ml |
| TEMED 0.004ml | TEMED 0.003ml |
| 10% APS 0.1 ml | 10% APS 0.03ml |
12 % SDS-PAGE
Separation gel mixture |
Stacking gel mixture |
| 1.5 M Tris pH8.8 2.5 ml | 1.0 M Tris pH6.8 0.38 ml |
| ddH2O 3.3 ml | ddH2O 2.1 ml |
| 10 % SDS 0.1 ml | 10 % SDS 0.03ml |
| 30 % Acrylamide 4.0 ml | 30 % Acrylamide 0.5 ml |
| TEMED 0.004ml | TEMED 0.003ml |
| 10% APS 0.1 ml | 10% APS 0.03ml |
筿溃100 V
Bio-Rad mini Gel Tank
蝴筿猘溅だ猂琌蹦ノLaemmli(1970)よ猭┮惠妓珇秈︽筿猘龟喷玡猰い礜だ牧瞒みよΑ埃馒借(1200rpm, 3だ牧)Bio-Radそbroad range leveled markerだ秖癸酚夹非ЧΘぇ0.1%Commassie brilliant blue R250琕︹ぼだ牧DestainI( 50% etthanol, 10% Acetic acid)科琕ぼだ牧Destain II(10% Acetic acid)科琕璉春︹癶