LOCUS 83686 639 aa 06-DEC-1996 DEFINITION glucan 1,4-alpha-glucosidase (EC 3.2.1.3) precursor - Aspergillus sp. ACCESSION 83686 PID g83686 DBSOURCE PIR: locus JQ0607 summary: #length 639 #molecular-weight 68130 #checksum 7684. genetic: #introns 72/1; 166/3; 199/1; 411/3. superfamily: Aspergillus glucan 1,4-alpha-glucosidase; glucan 1,4-alpha-glucosidase homology. PIR dates: 31-Mar-1992 #sequence_revision 31-Mar-1992 #text_change 06-Dec-1996. KEYWORDS glycosidase; hydrolase; polysaccharide degradation. SOURCE Aspergillus sp. ORGANISM Aspergillus sp. Eukaryotae; mitochondrial eukaryotes; Fungi; Ascomycota; Euascomycetes; Plectomycetes; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus. REFERENCE 1 (residues 1 to 639) AUTHORS Shibuya,I., Gomi,K., Iimura,Y., Takahashi,K., Tamura,G. and Hara,S. TITLE Molecular cloning of the glucoamylase gene of Aspergillus shirousami and its expression in Aspergillus oryzae JOURNAL Agric. Biol. Chem. 54 (8), 1905-1914 (1990) MEDLINE 91182400 COMMENT This enzyme catalyzes the release of glucose from the non-reducing ends of starch and related polysaccharides. FEATURES Location/Qualifiers source 1..639 /organism="Aspergillus sp." Protein 1..639 /note="glucoamylase" /product="glucan 1,4-alpha-glucosidase precursor" /EC_number="3.2.1.3" Region 1..24 /note="signal sequence" /region_name="domain" Region 23..446 /note="glucan 1,4-alpha-glucosidase homology #label GAG" /region_name="domain" Region 25..639 /note="glucoamylase" /region_name="product" ORIGIN 1 msfrsllals glvcsglasv iskratldsw lsneatvart ailnnigadg awvsgadsgi 61 vvaspstdnp dyfytwtrds givlktlvdl frngdtdlls tiehyissqa iiqgvsnpsg 121 dlssgglgep kfnvdetaya gswgrpqrdg palratamig fgqwlldngy tsaateivwp 181 lvrndlsyva qywnqtgydl weevngssff tiavqhralv egsafatavg sscswcdsqa 241 pqilcylqsf wtgsyilanf dssrsgkdtn tllgsihtfd peagcddstf qpcspralan 301 hkevvdsfrs iytlndglsd seavavgryp edsyyngnpw flctlaaaeq lydalyqwdk 361 qgsleitdvs ldffkalysg aatgtyssss styssivsav ktfadgfvsi vethaasngs 421 lseqfdksdg delsardltw syaalltann rrnsvvppsw getsassvpg tcaatsasgt 481 yssvtvtswp sivatggttt tatttgsggv tstsktttta sktstttsst scttptavav 541 tfdltattty geniylvgsi sqlgdwetsd gialsadkyt ssnppwyvtv tlpagesfey 601 kfirvesdds vewesdpnre ytvpqacges tatvtdtwr //