1. Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database. Give its

(a) accession number : P40692

(b) entry name : MLH1_HUMAN

(b) release date of last modification : Release 31, February 1995

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2. Give its number of amino acids, molecular weight and theoretical pI.

amino acids : 756aa

molecular weight : 84600.9Da

theoretical pI : 5.51

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3. Calculate the total number of negatively charged residues and positively charged residues.

Total number of negatively charged residues (Asp + Glu): 104

Total number of positively charged residues (Arg + Lys): 83

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4. Calculate its hydrophobicity (Kyte & Doolittle scale, window size 11, Relative weight 60%).

MIN: -3.443

MAX: 1.883

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5. Performe the trypsin (higher specificity) cleavage of the protein.

(a) How many peptides will you get after cleavage? 33

(b) Give the list of peptides with a mass bigger than 1000 dalton.

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