HOMEWORK7

1. Search human Mlh1 or its homologous proteins in Protein Data Bank (PDB) database. You just need to search for protein structure, do not find complex structure. Give its (a) ID number, (b) Name of the molecule, (c) experimental method for structure determination and (d) Resolution of the structure.

(a) ID number : 1BKN

(b) Name of the molecule : E. Coli DNA Mismatch Repair Protein Mutl

(c) Method : X-ray Diffraction

(d) Resolution : 2.90 A

2. Show its structure in ribbons form (400x400).

picture

3. Give the structural informations of this PDB file (a) number of protein chains, (b) number of helix in one protein chain, (c) the first and last residues in the helix 6 .

(a) 2 (A & B)

(b) A : 11 B : 10

(c) first : Thr last : Ala

4. Give the Class, Fold, Superfamily and Family of this protein in Structural Classification of Proteins (SCOP).

answer

5. (a) Show Ramachandran plot for this PDB file. (b) How many residues are in the most favoured regions of Ramachandran plot?

(a) plot

(b) 379 residues