HOMEWORK6
1. Add MLH1_Human protein to the Biology Workbench. Predict its secondary structure by GOR4.
2. Do a homology searching of MLH1_Human in Genpept Full Release Database. Import MLH1-like protein of C. elegans, S. cerevisiae, D. melanogaster, R. norvegicus and M. musculus to your workbench. Run CLUSTALW to get multiple sequence alignment for these six proteins.
3. Perform BOXSHADE program to get a color-coded plot for the results of question 2.
4. Draw rooted phylogenetic tree for these proteins.
T25389 hypothetical protein T28A8.7 - Caenorhabditis elegans
S43085 DNA mismatch repair protein MLH1 - human
GENPEPT:1724118 Rattus norvegicus mismatch repair protein (MLH1) mRNA, complete
GENPEPT:3192877 Drosophila melanogaster mutL homolog (Mlh1) gene, complete cds_
GENPEPT:7595954 Mus musculus MutL homolog 1 protein (MLH1) mRNA, complete cds.
GENPEPT:460627 Saccharomyces cerevisiae DNA mismatch repair (MLH1) gene, complete