HOMEWORK 3

1. How many hits will you get if you search genes associated with colon cancer in human genome?

→17 hits (human map viewer→search for colon cancer)

2. How many loci will you find if you search locus link for human in Genebank?

→21 loci(human genome resources→search locuslink for colon cancer)

3. Give the locus ID and position of MLH1.

→4292 ; 3p21.3

4. Find the %ID of nucleotide sequence for its possible orthologs in mouse.

→90.5 (human map viewer→search for MLH1→3 hits,choose map element:MLH1→ choose MLH1 in the map→click HOMOL→ choose the first oneand click "more"→see human/mouse)

5. Find the total number of mutations of MLH1 reported in human gene mutation database.

→147

6. Give the DNA sequence of MLH1.

→human map viewer→search for MLH1→3 hits,choose map element:MLH1→ choose MLH1 in the map→ choose RefSeq:Genbank:U07343 → sequence

7. Give the DNA sequence of E. coli mismatch repair gene mutS.

→neucleotide search for E. coli mismatch repair gene mutS→choose U69873→ sequence