Assignment 5

1.
(a) accession number: P40692
(b) entry name: MLH1_HUMAN
(c) release data of last modification:      sequence was last modified in February 1995, release 31
    annotations were last modified in October 2001, release 40

2.
Number of amino acids: 756 AA
Molecular weight: 84600 Da
Theoretical pI: 5.51

3.
Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys):  83

4.
MIN: -3.443
MAX: 1.883


5.
80peptides after cleavage
Peptides with a mass bigger than 1000 dalton: 
  mass    position #MC     peptide sequence 
4615.429  619-659  0   AEMLADYFSLEIDEEGNLIG LPLLIDNYVPPLEGLPIFIL R 
3115.482  576-604  0   LSEPAPLFDLAMLALDSPES GWTEEDGPK 
2862.478  287-311  0   NTHPFLYLSLEISPQNVDVN VHPTK 
2804.404  137-162  0   APPKPCAGNQGTQITVEDLF YNIATR 
2775.324  523-546  0   EMLHNHSFVGCVNPQWALAQ HQTK 
2751.311  689-713  0   QYISEESTLSGQQSEVPGSI PNSWK 
2634.410  500-522  0   IINLTSVLSLQEEINEQGHE VLR 
2625.413  393-416  0   LDAFLQPLSKPLSSQPQAIV TEDK 
2550.328  555-575  0   LSEELFYQILIYDFANFGVL R 
2201.124  733-751  0   HFTEDGNILQLANLPDLYK 
2140.082  342-361  0   MYFTQTLLPGLAGPSGEMVK  
1968.958  426-443  0   QQDEEMLELPAPAEVAAK 
1835.002  101-118  0   GEALASISHVAHVTITTK 
1833.902  85-100   0   LQSFEDLASISTYGFR 
1774.887  312-325  0   HEVHFLHEESILER 
1550.901  19-33    0   IAAGEVIQRPANAIK 
1532.692  362-377  0   STTSLTSSSTSGSSDK 
1525.596  475-487  0   EDSDVEMVEDDSR 
1460.684  242-254  0   MNGYISNANYSVK 
1410.751  605-616  0   EGLAEYIVEFLK 
1408.722  183-195  0   YSVHNAGISFSVK 
1338.730  275-286  0   AIETVYAAYLPK 
1333.627  660-670  0   LATEVNWDEEK 
1327.733  58-69    0   LIQIQDNGTGIR 
1300.686  206-217  0   TLPNASTVDNIR 
1275.703  256-265  0   CIFLLFINHR 
1174.625  714-722  0   WTVEHIVYK 
1165.523  34-43    0   EMIENCLDAK 
1148.550  227-236  0   ELIEIGCEDK 
1119.496  679-687  0   ECAMFYSIR 
1092.517  444-453  0   NQSLEGDTTK 
1091.504  71-79    0   EDLDIVCER 
1003.514  378-385  0   VYAHQMVR