ProtParam tool
DE DNA mismatch repair protein Mlh1 (MutL protein homolog 1).
OS Homo sapiens (Human).
The computation has been carried out on the
complete sequence.
Warning: All computation results shown below do not take into account
any annotated post-translational modification.References and documentation are available.
Number of amino acids: 756
Molecular weight: 84600.9
Theoretical pI: 5.51
Amino acid composition:
Ala (A) 50 6.6%
Arg (R) 36 4.8%
Asn (N) 33 4.4%
Asp (D) 34 4.5%
Cys (C) 11 1.5%
Gln (Q) 30 4.0%
Glu (E) 70 9.3%
Gly (G) 40 5.3%
His (H) 19 2.5%
Ile (I) 48 6.3%
Leu (L) 76 10.1%
Lys (K) 47 6.2%
Met (M) 14 1.9%
Phe (F) 26 3.4%
Pro (P) 34 4.5%
Ser (S) 69 9.1%
Thr (T) 45 6.0%
Trp (W) 5 0.7%
Tyr (Y) 23 3.0%
Val (V) 46 6.1%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 83
Atomic composition:
Carbon C 3740
Hydrogen H 5947
Nitrogen N 1017
Oxygen O 1165
Sulfur S 25
Formula: C3740H5947N1017O1165S25
Total number of atoms: 11894
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 61075 60835 59835 58490 56200
Abs 0.1% (=1 g/l) 0.722 0.719 0.707 0.691 0.664
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 60350 60200 59235 57890 55600
Abs 0.1% (=1 g/l) 0.713 0.712 0.700 0.684 0.657
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.29
This classifies the protein as unstable.
Aliphatic index: 88.23
Grand average of hydropathicity (GRAVY): -0.353