1 aactgcaaat tgccggacag atctgcctgt ccggcatact attcatgagg
ttttttcgga
61 cgatattttt ccggcagttc tggcaccgga cgcttgtcat cgatgagatg acgcacggtt
121 aagatcggat gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct
181 tcacgctttg caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg
241 ccgaacacac atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc
301 ggctccgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
361 agtttttcac gagagatacg cttgccggac atgctgcctc cacctcatta agatgtattt
421 atattacatc ttaatcttaa agggcactat gactccaaag aagaagggtt agccaaccga
481 tacaattttg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
541 cggaaccagc atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat
601 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
661 ggaaccggac ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
721 cagcagtatc tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
781 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
841 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
901 gcggtggaaa actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa
961 caaattggcg atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
1021 acgccaggca ccatcagcga tgaagccctg ttgcaggagc gtcaggacaa cctgctggcg
1081 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ttccgggcgt
1141 tttcgcctga gcgaaccggc tgaccgcgaa acgatggcgg cagaactgca acgcactaat
1201 cctgcggaac tgctgtatgc agaagatttt gctgaaatgt cgttaattga aggccgtcgc
1261 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
1321 ctgcaatttg ggacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
1381 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgtacgac tctgccgcat
1441 attcgttcca tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacgcgt
1501 cgtaatctgg aaatcaccca gaacctggcg ggtggtgcgg aaaatacgct ggcttctgtg
1561 ctcgactgca ccgtcacgcc gatgggcagc cgtatgctga aacgctggct gcatatgcca
1621 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc
1681 accgccgggc tacagccggt actgcgtcag gtcggcgacc tggaacgtat tctggcacgt
1741 ctggctttac gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa
1801 ctgccggagc tgcgtgcgca gttagaaact gtcgatagtg caccggtaca ggcgctacgt
1861 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
1921 ccgccggtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat
1981 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
2041 cgtgaacgta ccggcctgga cacgctgaaa gttggcttta atgcggtgca cggctactac
2101 attcaaatca gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg
2161 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc
2221 acctcaaaag gcaaagcact ggcactggaa aaacagcttt atgaagagct gttcgacctg
2281 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
2341 ctggttaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat
2401 aaaccgggca ttcgcattac cgaaggtcgc catccggtag ttgaacaagt actgaatgag
2461 ccatttatcg ccaacccgct gaatctgtcg ccgcagcgcc gcatgttgat catcaccggt
2521 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cactgattgc gctgatggcc
2581 tacatcggca gctatgtacc ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt
2641 acccgcgtag gcgcggcaga tgacctggcg tccgggcgct caacctttat ggtggagatg
2701 actgaaaccg ccaatatttt acataacgcc accgaataca gtctggtgtt aatggatgag
2761 atcgggcgtg gaacgtccac ctacgatggt ctgtcgctgg cgtgggcgtg cgcggaaaat
2821 ctggcgaata agattaaggc attgacgtta tttgctaccc actatttcga gctgacccag
2881 ttaccggaga aaatggaagg cgtcgctaac gtgcatctcg atgcactgga gcacggcgac
2941 accattgcct ttatgcacag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg
3001 gttgcagctc tggcaggcgt gccaaaagag gttattaagc gcgcacggca aaagctgcgt
3061 gagctggaaa gcatttcgcc gaacgccgcc gctacgcaag tggatggtac gcaaatgtct
3121 ttgctgtcag taccagaaga aacttcgcct gcggtcgaag ctctggaaaa tcttgatccg
3181 gattcactca ccccgcgtca ggcgctggag tggatttatc gcttgaagag cctggtgtaa
3241 taacaattcc cgatagtctt ttgctatcgg gaatattaac gacaactgac gaataaaata
3301 aaaacaccct gtataatagg aaagctt
Assignment
3
Search
genes associated with colon cancer in human genome.
MLH1 was identified as a locus
frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned,
it was discovered to be a human homolog of the E. coli mismatch repair gene
mutS.
1. How many hits will you get if you search genes associated with colon cancer in human genome?
Ans :
2020
2. How many loci will you find if you search locus link for human in Genebank?
Ans : 21
3. Give the locus ID and position of MLH1.
Ans : Locus
ID : 4292 . Position
: 3p21.3
4. Find the %ID of nucleotide sequence for its possible orthologs in mouse.
Ans :
90.5 %
5. Find the total number of mutations of MLH1 reported in human gene mutation database.
Ans : 147
6. Give the DNA sequence of MLH1.
Ans : BASE
COUNT
7. Give the DNA sequence of E. coli mismatch repair gene mutS.
Ans : BASE COUNT