AlignMaster output
============================================================

Length of target sequence: 756 residues

Reading user-defined template list

Extracting template sequences

Running pair-wise alignments with target sequence
Sequence identity of templates with target:

1BKNA.pdb: 46.05 % identity
1BKNB.pdb: 40.9 % identity

Looking for template groups
Global alignment overview:

Taget Sequence: |==================================================|
1BKNA.pdb | -------
1BKNB.pdb | -------

AlignMaster found 1 regions to model separately:
1: Using template(s) 1BKNA.pdb 1BKNB.pdb

1BKNA
CRYSTAL STRUCTURE OF AN N-TERMINAL 40KD FRAGMENT OF E. COL
DNA MISMATCH REPAIR PROTEIN MUTL
MOL_ID: 1;
MOLECULE: MUTL;
CHAIN: A, B;
FRAGMENT: N-TERMINAL 40KD FRAGMENT;
ENGINEERED: YES
MOL_ID: 1;
ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;
STRAIN: K-12;
PLASMID: PTX418;
GENE: MUTL;
EXPRESSION_SYSTEM: ESCHERICHIA COLI;
EXPRESSION_SYSTEM_STRAIN: HMS174 (LAMBDA DE3);
EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
EXPRESSION_SYSTEM_PLASMID: PTX418;
EXPRESSION_SYSTEM_GENE: MUTL
W.YANG,C.BAN

Length = 272

Score = 124 bits (309), Expect = 1e-28
Identities = 81/287 (28%) Positives = 144/287 (49%) Gaps = 30/287 (10%)

Query: 25  IQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSK 84
             RPA   KE  EN LDA  T I      GG KLI I DNG GI K  L           
Sbjct: 1   VERPASVVKELVENSLDAGATRIDIDIERGGAKLIRIRDNGCGIKKDELALAL------A 54

   
Query: 85  LQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAG 144
             S  DL  I   GFRGEALASIS V   T T  TA    A                   
Sbjct: 55  IASLDDLEAIISLGFRGEALASISSVSRLTLTSRTAEQQEAW------------------ 96

Query: 145 NQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADV 204
            Q    TV        T    L     E   I E   R               G  V   
Sbjct: 97  -QAYAVTVKPAAHPVGTTLEVLDTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQY 155

Query: 205 RTLPNASTVD-NIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKC--IFLLF 261
           R  P           I G A       I      L     G     N        I    
Sbjct: 156 RAVPEGGQKERRLGAICGTAFLEQALAIEWQHGDLT--LRGWVADPNHTTPALAEIQYCY 213

Query: 262 INHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVNVH 308
            N R         AI       L     P   L LEI P  V   VH
Sbjct: 214 VNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVSRLVH 260


1BKNB 
CRYSTAL STRUCTURE OF AN N-TERMINAL 40KD FRAGMENT OF E. COL
DNA MISMATCH REPAIR PROTEIN MUTL                         
MOL_ID: 1;                                                
MOLECULE: MUTL;                                          
CHAIN: A, B;                                             
FRAGMENT: N-TERMINAL 40KD FRAGMENT;                      
ENGINEERED: YES                                          
MOL_ID: 1;                                                
ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                   
STRAIN: K-12;                                            
PLASMID: PTX418;                                         
GENE: MUTL;                                              
EXPRESSION_SYSTEM: ESCHERICHIA COLI;                     
EXPRESSION_SYSTEM_STRAIN: HMS174 (LAMBDA DE3);           
EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                  
EXPRESSION_SYSTEM_PLASMID: PTX418;                       
EXPRESSION_SYSTEM_GENE: MUTL                             
W.YANG,C.BAN 

Length = 271
           
 Score =  118 bits (293), Expect = 8e-27
 Identities = 81/282 (28%) Positives = 141/282 (49%) Gaps = 33/282 (11%)

Query: 25  IQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQIQDNGTGIRKEDLDIVCERFTTSK 84
             RPA   KE  EN LDA  T I      GG KLI I DNG GI K  L           
Sbjct: 1   VERPASVVKELVENSLDAGATRIDIDIERGGAKLIRIRDNGCGIKKDELALALAE----- 55

Query: 85  LQSFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAG 144
                    I   GFRGEALASIS V   T T  TA    A  A           KP A 
Sbjct: 56  --------AIISLGFRGEALASISSVSRLTLTSRTAEQQEAWQA-----YMNVTVKPAAH 102

Query: 145 NQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADV 204
             GT   V DL          L     E   I E   R               G  V   
Sbjct: 103 PVGTTLEVLDL----------LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQY 152

Query: 205 RTLPNASTVD-NIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKC--IFLLF 261
           R  P           I G A       I      L     G     N        I    
Sbjct: 153 RAVPEGGQKERRLGAICGTAFLEQALAIEWQHGDLT--LRGWVADPNHTTPALAEIQYCY 210

Query: 262 INHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNV 303
            N R         AI       L     P   L LEI P  V
Sbjct: 211 VNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQV 252

2. How large is the fragment of MLH1 possible to be modeled?

   336 residues

3. Show three rasmol pictures of your model of MLH1?