1. Search human MutL protein homolog 1, Mlh1, in SWISS-PROT database. Give its (a) accession number, (b) entry name and (b) release date of last modification.

(a) accesstion number: P40692
(b) entry name: MLH1_HUMAN
(c) release date of last modification: October 2001

How to find: SwissProt --> Quick search for Mlh1 --> select Mlh1 Human
Click to see
           

2. Give its number of amino acids, molecular weight and theoretical pI.

Number of amino acids: 756 
Molecular weight: 84600.98 Dalton
Theoretical pI: 5.51

How to find: From 1., go down to the bottom of the page, choose "calculate pI/MW" in Sequence Analysis Tools --> Submit
Click to see

3. Calculate the total number of negatively charged residues and positively charged residues.

Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 83

How to find: Choose Protparam in tools --> Submit
Click to see

4. Calculate its hydrophobicity (Kyte & Doolittle scale, window size 11, Relative weight 60%).

          Weights for window positions 1,..,11, using linear weight variation model:
           1       2      3       4       5      6       7      8       9     10     11    
         0.60  0.68  0.76  0.84  0.92  1.00  0.92  0.84  0.76  0.68  0.60 
         edge                                  center                                 edge

How to find: Choose Protscale in tools --> select Hphob./ Kyte & Doolittle, window size 11, relative weight 60% --> 
                    submit --> submit again
Click to see

5.Performe the trypsin (higher specificity) cleavage of the protein. 
(a) How many peptides will you get after cleavage? 
(b) Give the list of peptides with a mass bigger than 1000 dalton.

(a) 33 peptides after cleavage
(b) Click here to see list

How to find: Choose PeptideMass in tools --> Select enzyme as Trypsin (higher specificity), select Display the peptides 
                    with a mass bigger than 1000 Dalton --> Perform Cleavage