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HOMEWORK 6

1. Please help him to identify this gene (name, accession #, authors ...... )

Ans: Take a look!

2. Which organism does this gene belong?

Ans: Tetrahymena pyriformis

3. How many nucleotide, protein and structure have been known for this organism?

Ans:

nucleotide
95ºØ
protein
231ºØ
structure
0ºØ


 

 

4. Do the Blast search for this gene. List the top 10 most similar sequence.

Ans:
Sequences producing significant alignments: Score(bits), E-Value


(1)dbj|D13920.1|TETHEMOGP Tetrahymena pyriformis mRNA for hemo... 1148 0.0
(2)dbj|D13919.1|TETHEMOGT Tetrahymena thermophila mRNA for hem... 70 1e-09
(3)emb|AL049870.3|CNS0000S Human chromosome 14 DNA sequence **... 48 0.005
(4)gb|U56081.1|CEU56081 Caenorhabditis elegans T-BOX 12 (Ce-tb... 48 0.005
(5)gb|AF194867.1|AF194867 Portulaca oleracea NADH dehydrogenas... 46 0.019
(6)gb|AF194854.1|AF194854 Portulaca molokiniensis NADH dehydro... 46 0.019
(7)gb|AF194853.1|AF194853 Portulaca grandiflora NADH dehydroge... 46 0.019
(8)emb|AJ235828.1|MBR235828 Mostuea brunonis chloroplast ndhF ... 46 0.019
(9)gb|AF130178.1|AF130178 Montinia caryophyllacea NADH dehydro... 44 0.076
(10_gb|AE003647.1|AE003647 Drosophila melanogaster genomic scaf... 40 1.2


5. Using ORF finder to translate this gene. Show the correct protein sequence.

Ans:

1 mnkpqtiyek lggenamkaa vplfykkvla dervkhffkn tdmdhqtkqq tdfltmllgg

61 pnhykgknmt eahkgmnlqn lhfdaiienl aatlkelgvt davineaakv iehtrkdmlg

121 k