Version 3.2
Selected Sequence(s) no.1 Q10784. HEMOGLOBIN HBN. Mycobacterium tuberculosis, and Mycobacterium bovis. P15160. MYOGLOBIN (HEMOGLOBIN). Paramecium caudatum. Tetrahymena thermophila mRNA for hemoglobin. Q03459. MYOGLOBIN (HEMOGLOBIN). Tetrahymena thermophila. Tetrahymena pyriformis mRNA for hemoglobin. P17724. MYOGLOBIN (HEMOGLOBIN). Tetrahymena pyriformis.
Consensus key (see documentation for details) * - single, fully conserved residue : - conservation of strong groups . - conservation of weak groups - no consensus CLUSTAL W (1.81) multiple sequence alignment GLB_TETTH --------MRKQPTVFEKLGGQAAMHAAVPLFYKKVLADDRVKHYFKNTNMEHQAKQQED TETHEMOGT_418107 --------MRKQPTVFEKLGGQAAMHAAVPLFYKKVLADDRVKHYFKNTNMEHQAKQQED GLB_TETPY --------MNKPQTIYEKLGGENAMKAAVPLFYKKVLADERVKHFFKNTDMDHQTKQQTD TETHEMOGP_418106 --------MNKPQTIYEKLGGENAMKAAVPLFYKKVLADERVKHFFKNTDMDHQTKQQTD no.1 --------MNKPQTIYEKLGGENAMKAAVPLFYKKVLADERVKHFFKNTDMDHQTKQQTD GLB_PARCA -------------SLFEQLGGQAAVQAVTAQFYANIQADATVATFFNGIDMPNQTNKTAA GLBN_MYCTU MGLLSRLRKREPISIYDKIGGHEAIEVVVEDFYVRVLADDQLSAFFSGTNMSRLKGKQVE ::::::**. *:.... ** .: ** : :*.. :* . : GLB_TETTH FLTMLLGGPNHYKGKNMAEAHKGMNLQNSHFDAIIENLAATLKELGVSDQIIGEAAKVIE TETHEMOGT_418107 FLTMLLGGPNHYKGKNMAEAHKGMNLQNSHFDAIIENLAATLKELGVSDQIIGEAAKVIE GLB_TETPY FLTMLLGGPNHYKGKNMTEAHKGMNLQNLHFDAIIENLAATLKELGVTDAVINEAAKVIE TETHEMOGP_418106 FLTMLLGGPNHYKGKNMTEAHKGMNLQNLHFDAIIENLAATLKELGVTDAVINEAAKVIE no.1 FLTMLLGGPNHYKGKNMTEAHKGMNLQNLHFDAIIENLAATLKELGVTDAVINEAAKVIE GLB_PARCA FLCAALGGPNAWTGRNLKEVHANMGVSNAQFTTVIGHLRSALTGAGVAAALVEQTVAVAE GLBN_MYCTU FFAAALGGPEPYTGAPMKQVHQGRGITMHHFSLVAGHLADALTAAGVPSETITEILGVIA *: ****: :.* : :.* . .: :* : :* :*. **. : : * GLB_TETTH HTRKDCLGK------- TETHEMOGT_418107 HTRKDCLGK------- GLB_TETPY HTRKDMLGK------- TETHEMOGP_418106 HTRKDMLGK------- no.1 HTRKDMLGK------- GLB_PARCA TVRGDVVTV------- GLBN_MYCTU PLAVDVTSGESTTAPV *
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( ( GLB_TETPY:0.00000, TETHEMOGP_418106:0.00000) :0.00000, ( ( GLB_TETTH:0.00000, TETHEMOGT_418107:0.00000) :0.09306, ( GLB_PARCA:0.31439, GLBN_MYCTU:0.33216) :0.25009) :0.08049, no.1:0.00000);
Alignment type: Protein Alignment order: aligned Pairwise alignment parameters Method: accurate Matrix: Gonnet Gap open pentalty: 10.00 Gap extension pentalty: 0.10 Multiple alignment parameters Matrix: Gonnet Negative matrix?: no Gap open pentalty: 10.00 Gap extension pentalty: 0.20 % identity for delay: 30 Residue-specific gap penalties: on Penalize end gaps: on Hydrophylic gap penalties: on Gap separation distance: 0 Hydrophylic residues: GPSNDQEKR CLUSTAL W (1.81) Multiple Sequence Alignments Sequence type explicitly set to Protein Sequence format is Pearson Sequence 1: GLB_TETPY 121 aa Sequence 2: TETHEMOGP_418106 121 aa Sequence 3: GLB_TETTH 121 aa Sequence 4: TETHEMOGT_418107 121 aa Sequence 5: GLB_PARCA 116 aa Sequence 6: GLBN_MYCTU 136 aa Sequence 7: no.1 121 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 100 Sequences (1:3) Aligned. Score: 82 Sequences (1:4) Aligned. Score: 82 Sequences (1:5) Aligned. Score: 35 Sequences (1:6) Aligned. Score: 33 Sequences (1:7) Aligned. Score: 100 Sequences (2:3) Aligned. Score: 82 Sequences (2:4) Aligned. Score: 82 Sequences (2:5) Aligned. Score: 35 Sequences (2:6) Aligned. Score: 33 Sequences (2:7) Aligned. Score: 100 Sequences (3:4) Aligned. Score: 100 Sequences (3:5) Aligned. Score: 34 Sequences (3:6) Aligned. Score: 32 Sequences (3:7) Aligned. Score: 82 Sequences (4:5) Aligned. Score: 34 Sequences (4:6) Aligned. Score: 32 Sequences (4:7) Aligned. Score: 82 Sequences (5:6) Aligned. Score: 35 Sequences (5:7) Aligned. Score: 35 Sequences (6:7) Aligned. Score: 33 Guide tree file created: [../tmp-dir/22846.CLUSTALW.dnd] Start of Multiple Alignment There are 6 groups Aligning... Group 1: Sequences: 2 Score:2630 Group 2: Sequences: 2 Score:2622 Group 3: Sequences: 3 Score:2622 Group 4: Sequences: 5 Score:2428 Group 5: Sequences: 6 Score:1741 Group 6: Sequences: 7 Score:1436 Alignment Score 9206 CLUSTAL-Alignment file created [../tmp-dir/22846.CLUSTALW.aln]
Citation
Algorithm Citation:
Higgins, D.G., Bleasby, A.J. and Fuchs, R. (1992) CLUSTAL V: improved software for multiple sequence alignment. Computer Applications in the Biosciences (CABIOS), 8(2):189-191.
Thompson J.D., Higgins D.G., Gibson T.J. "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice." Nucleic Acids Res. 22:4673-4680(1994).
Felsenstein, J. 1989. PHYLIP -- Phylogeny Inference Package (Version 3.2). Cladistics 5: 164-166.
Program Citation:
CLUSTAL W: Julie D. Thompson, Desmond G. Higgins and Toby J. Gibson, modified; any errors are due to the modifications.
PHYLIP: Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle.
Copyright (C) 1999, Board of Trustees of the University of Illinois.