ProtParam tool
User-provided sequence:
1 11 21 31 41 51
| | | | | |
1 MNKPQTIYEK LGGENAMKAA VPLFYKKVLA DERVKHFFKN TDMDHQTKQQ TDFLTMLLGG 60
61 PNHYKGKNMT EAHKGMNLQN LHFDAIIENL AATLKELGVT DAVINEAAKV IEHTRKDMLG 120
121 K
References
and documentation are available.
Number of amino acids:
121
Molecular weight: 13683.8
Theoretical pI: 8.78
Amino acid composition:
Ala (A) 11 9.1%
Arg (R) 2 1.7%
Asn (N) 9 7.4%
Asp (D) 7 5.8%
Cys (C) 0 0.0%
Gln (Q) 5 4.1%
Glu (E) 8 6.6%
Gly (G) 8 6.6%
His (H) 6 5.0%
Ile (I) 5 4.1%
Leu (L) 12 9.9%
Lys (K) 15 12.4%
Met (M) 7 5.8%
Phe (F) 5 4.1%
Pro (P) 3 2.5%
Ser (S) 0 0.0%
Thr (T) 9 7.4%
Trp (W) 0 0.0%
Tyr (Y) 3 2.5%
Val (V) 6 5.0%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 17
Atomic composition:
Carbon C 606
Hydrogen H 972
Nitrogen N 168
Oxygen O 178
Sulfur S 7
Formula: C606H972N168O178S7
Total number of atoms: 1931
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 4350 4200 4035 3840 3600
Abs 0.1% (=1 g/l) 0.318 0.307 0.295 0.281 0.263
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 25.91
This classifies the protein as stable.
Aliphatic index: 78.26
Grand average of hydropathicity (GRAVY): -0.545