ProtParam tool

User-provided sequence:

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GAAAAAAATA AAGAAAATGA ACAAACCCCA AACTATTTAT GAAAAGCTCG GAGGCGAAAA    60
   61 TGCCATGAAG GCTGCCGTCC CTCTCTTCTA CAAGAAGGTC TTAGCTGATG AAAGAGTCAA   120
  121 GCATTTCTTC AAGAACACCG ACATGGATCA CCAAACCAAG CAATAAACTG ACTTCCTCAC   180
  181 CATGCTCTTA GGTGGTCCCA ACCATTACAA GGGTAAAAAT ATGACTGAAG CTCACAAGGG   240
  241 TATGAACTTG CAAAACTTGC ACTTTGATGC CATCATTGAA AACCTTGCTG CTACCCTTAA   300
  301 GGAGCTCGGT GTCACCGATG CTGTTATTAA CGAGGCTGCT AAGGTCATCG AACACACCCG   360
  361 TAAGGATATG CTCGGCAAGT GAGATAGCTG CTGTTGCTGT TTATATTCTA CTATTATTAA   420
  421 TTACTACTTA ACACATCATC AAATAAATAG TAATTCTACT CAATTAAACT TGTCAGATTT   480
  481 CAATAAAAAT TATTTACTGT AATGAGAGTA TTTATTGTGT ATTGTTATGT ATCGTTTATT   540
  541 GAAGATGATG ATCAAGACAA ATCCCATGGT ACCCGATCCT CGAATTC

Number of amino acids: 587

Molecular weight: 49086.0

Theoretical pI: 5.18

Amino acid composition:

Ala (A) 198	 33.7%
Arg (R)   0	  0.0%
Asn (N)   0	  0.0%
Asp (D)   0	  0.0%
Cys (C) 121	 20.6%
Gln (Q)   0	  0.0%
Glu (E)   0	  0.0%
Gly (G) 104	 17.7%
His (H)   0	  0.0%
Ile (I)   0	  0.0%
Leu (L)   0	  0.0%
Lys (K)   0	  0.0%
Met (M)   0	  0.0%
Phe (F)   0	  0.0%
Pro (P)   0	  0.0%
Ser (S)   0	  0.0%
Thr (T) 164	 27.9%
Trp (W)   0	  0.0%
Tyr (Y)   0	  0.0%
Val (V)   0	  0.0%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 0
Total number of positively charged residues (Arg + Lys): 0

Atomic composition:

Carbon      C	      1821
Hydrogen    H	      3057
Nitrogen    N	       587
Oxygen      O	       752
Sulfur      S	       121

Formula: C1821H3057N587O752S121
Total number of atoms: 6338

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient     8700    7620    7200    7200    7200
Abs 0.1% (=1 g/l)   0.177   0.155   0.147   0.147   0.147



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient        0       0       0       0       0
Abs 0.1% (=1 g/l)   0.000   0.000   0.000   0.000   0.000


Estimated half-life:

The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.44
This classifies the protein as unstable.



Aliphatic index: 33.73

Grand average of hydropathicity (GRAVY): 0.856