Using
MASCOT to search possible protein identity
(http://www.matrixscience.com)
| 1. get your sample
peptide data from IBC core facility (MALDI-Q-TOF or ESI-Q-TOF) 2. key in (http://www.matrixscience.com) 3. Choose MASCOT 4. You can choose Peptide Mass Fingerprint (MALDI-Q-TOF)or MS/MS Ion Search (LC MS-MS) |
| Peptide Mass Fingerprint (MALDI-Q-TOF) |
 |
| MS/MS Ion Search (LC MS-MS) |
 |
| 5. Analyze your
search result.
Here is an example: |
 |
| The hit protein
will be grouped when you get your search result. When you hit the button of your interested protein, you will get more information about the alignment of your peptides. (For example: Q15459) |
|
|
| Here you can hit the Q15452 button to get the detailed information of this protein |
 |
| In this page, you can get the information of peptide alignment, sequence coverage, and the predicted peptides of this protein. |
