Midterm
PART I
a) What is the cytogenetic position of p53 in the CytogeneticMap?
17p13.1
b) Which chromosome is this gene located ?
chromosome 17
c) What is its LocusID ?
7157
d) What is the percent identity
H. sapiens 100%/392a.a
M. musculus 77%/395a.a
R.norvegicus 77%/392a.a
e) Find a paper that identified p53 as a sequence-specific DNA-binding protein. The authors are in the department of pathology, Johns Hopkins University of Medicine. Give the name of Journal, Vol. #, page # and year.
Science 1991,Jun 21
Vol 252(5013) page1708-11
f) Show the protein sequence of p53. (in fasta format)
g) Find the PDB ID
crystal structure of p53 tetramerization domain :1C26
core domain with DNA:1TSR
PART II
1.asp32 his64 ser33
ser37 his39 asp36