Homework 7
1. Using DNA -> Protein translator to translate gene.
Ans:
M N K P Q T I Y E K L G G E N A M K A A V P L F Y K K V L A D E R V K H F F K N T D M D H Q T K Q Q T D F L T M L L G G P N H Y K G K N M T E A H K G M N L Q N L H F D A I I E N L A A T L K E L G V T D A V I N E A A K V I E H T R K D M L G K Stop
121 a.a
2. Calaulate pI, molecular weight, amino acid composition of the protein.
Ans:
Number of amino acids: 121
Molecular weight: 13683.8
Theoretical pI: 8.78
Amino acid composition: Ala (A) 11 9.1% Arg (R) 2 1.7% Asn (N) 9 7.4% Asp (D) 7 5.8% Cys (C) 0 0.0% Gln (Q) 5 4.1% Glu (E) 8 6.6% Gly (G) 8 6.6% His (H) 6 5.0% Ile (I) 5 4.1% Leu (L) 12 9.9% Lys (K) 15 12.4% Met (M) 7 5.8% Phe (F) 5 4.1% Pro (P) 3 2.5% Ser (S) 0 0.0% Thr (T) 9 7.4% Trp (W) 0 0.0% Tyr (Y) 3 2.5% Val (V) 6 5.0% Asx (B) 0 0.0% Glx (Z) 0 0.0% Xaa (X) 0 0.0%
3. Predict a-helice of the protein.
Ans:
4. Compute the hydrophobic profile of the protein (show the window and weight you used).
Ans:
click here to see the page( This is from the Kyte & Doolittle model,and the window size is 9,and the relative weight of the window edges compared to the window center is 100%.)
