Homework 6
1. Please help him to identify this gene (name, accession #, authors ...... )
Ans:
LOCUS TETHEMOGP 587 bp mRNA INV 27-OCT-2000
DEFINITION Tetrahymena pyriformis mRNA for hemoglobin.
ACCESSION D13920
VERSION D13920.1 GI:217409
KEYWORDS hemoglobin.
SOURCE Tetrahymena pyriformis cDNA to mRNA.
ORGANISM Tetrahymena pyriformis
Eukaryota; Alveolata; Ciliophora; Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymena.
AUTHORS Takagi,T., Iwaasa,H., Yuasa,H., Shikama,K., Takemasa,T. and Watanabe,Y.
TITLE Primary structure of Tetrahymena hemoglobins
2. Which organism does this gene belong?
Ans:Tetrahymena pyriformis
3. How many nucleotide, protein and structure have been known for this organism?
Ans: 95 nucliotide ; 231 proteins
4. Do the Blast search for this gene. List the top 10 most similar sequence.
Ans:
(1)dbj|D13919.1|TETHEMOGT Tetrahymena thermophila mRNA for hem... 70 1e-09
(2)emb|AL049870.3|CNS0000S Human chromosome 14 DNA sequence **... 48 0.005
(3)gb|U56081.1|CEU56081 Caenorhabditis elegans T-BOX 12 (Ce-tb... 48 0.005
(4)gb|AF194867.1|AF194867 Portulaca oleracea NADH dehydrogenas... 46 0.019
(5)gb|AF194854.1|AF194854 Portulaca molokiniensis NADH dehydro... 46 0.019
(6)gb|AF194853.1|AF194853 Portulaca grandiflora NADH dehydroge... 46 0.019
(7)emb|AJ235828.1|MBR235828 Mostuea brunonis chloroplast ndhF ... 46 0.019
(8)gb|AF130178.1|AF130178 Montinia caryophyllacea NADH dehydro... 44 0.077
(9)gb|AE003647.1|AE003647 Drosophila melanogaster genomic scaf... 40 1.2
(10)gb|AE003412.1|DROSADH06 Drosophila melanogaster, chromosome... 40 1.2
5. Using ORF finder to translate this gene. Show the correct protein sequence.
Ans: ( Here you can see sixframes )
translation=MNKPQTIYEKLGGENAMKAAVPLFYKKVLADERVKHFFKNTDMD
HQTKQQTDFLTMLLGGPNHYKGKNMTEAHKGMNLQNLHFDAIIENLAATLKELGVTDA
VINEAAKVIEHTRKDMLGK