Homework 6

1. Please help him to identify this gene (name, accession #, authors ...... )

Ans:

LOCUS TETHEMOGP 587 bp mRNA INV 27-OCT-2000

DEFINITION Tetrahymena pyriformis mRNA for hemoglobin.

ACCESSION D13920

VERSION D13920.1 GI:217409

KEYWORDS hemoglobin.

SOURCE Tetrahymena pyriformis cDNA to mRNA.

ORGANISM Tetrahymena pyriformis

Eukaryota; Alveolata; Ciliophora; Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymena.

AUTHORS Takagi,T., Iwaasa,H., Yuasa,H., Shikama,K., Takemasa,T. and Watanabe,Y.

TITLE Primary structure of Tetrahymena hemoglobins

( 詳細資料 )

2. Which organism does this gene belong?

Ans:Tetrahymena pyriformis

3. How many nucleotide, protein and structure have been known for this organism?

Ans: 95 nucliotide ; 231 proteins

4. Do the Blast search for this gene. List the top 10 most similar sequence.

Ans:

(1)dbj|D13919.1|TETHEMOGT Tetrahymena thermophila mRNA for hem... 70 1e-09

(2)emb|AL049870.3|CNS0000S Human chromosome 14 DNA sequence **... 48 0.005

(3)gb|U56081.1|CEU56081 Caenorhabditis elegans T-BOX 12 (Ce-tb... 48 0.005

(4)gb|AF194867.1|AF194867 Portulaca oleracea NADH dehydrogenas... 46 0.019

(5)gb|AF194854.1|AF194854 Portulaca molokiniensis NADH dehydro... 46 0.019

(6)gb|AF194853.1|AF194853 Portulaca grandiflora NADH dehydroge... 46 0.019

(7)emb|AJ235828.1|MBR235828 Mostuea brunonis chloroplast ndhF ... 46 0.019

(8)gb|AF130178.1|AF130178 Montinia caryophyllacea NADH dehydro... 44 0.077

(9)gb|AE003647.1|AE003647 Drosophila melanogaster genomic scaf... 40 1.2

(10)gb|AE003412.1|DROSADH06 Drosophila melanogaster, chromosome... 40 1.2

5. Using ORF finder to translate this gene. Show the correct protein sequence.

Ans: ( Here you can see sixframes )

translation=MNKPQTIYEKLGGENAMKAAVPLFYKKVLADERVKHFFKNTDMD

HQTKQQTDFLTMLLGGPNHYKGKNMTEAHKGMNLQNLHFDAIIENLAATLKELGVTDA

VINEAAKVIEHTRKDMLGK