Material : pM/PKR, pVP16/2A and report(luciferase plasmids); BHK-21

Method : mammalian two hybrid assay system

pVP16/P2A : subcloning

                                             Double digestion ( BamH1+Xba1)

                                                      autosequencing

                                                                 inframe  

pM/PKR : clonging PCR product

                    PCR amplification     Double digestion ( BamH1+Xba1)         

                    Double digestion ( BamH1+Xba1 )         Cip