hk7

Homework #7

Jason Lee, a student in the department of Life Sciences,

got the mRNA from a plant.

Here is the sequence:

    
        1 atccactact tcatcataaa cctcacaact actattctat cttctcttct ctaattttca
       61 taatcattaa gaatggaaat ggttaacaag attgcatgct ttgtgctttt atgcatggta
      121 gtggttgcac cccatgcaga ggcactaact tgtggtcaag ttacatctac cttggctcct
      181 tgtctccctt atctaatgaa tcgcggtcct ctcggaggct gttgtggtgg tgttaagggt
      241 cttttgggtc aagcccagac tacagtagac cgacagaccg catgcacttg cctaaaatca
      301 gctgcttctt cttttacagg ccttgatttg ggcaaagctg ctagtctccc tagcacttgt
      361 agtgtcaaca tcccttacaa gatcagcccc tctactgact gctctaaagt tcagtaaagc
      421 tgatcatcag aatttggttt catgaggaga attaagaata agatagatag cattgatctt
      481 gcttatggat cctttctttc tatgttgtat cagttgtcac tttctgtttt ttctgtgttt
      541 cctttaaatt ctcgtatgta gtcgagtctt gtatcgaaat ttgacgattg attatattgt
      601 atcagttgtt actttctgtt ttcctgtgtt tcttttaaaa tcgtatgtag tcgagtcttg
      661 tatcgaaatt tcccgattgg ctatgttgta ttaatctaat ctttgataat acacatctat
      721 cttatttggt

1. Please help him to translate the DNA sequence to protein sequence.

SQ SEQUENCE 114 AA; 11716 MW; EA5981F6 CRC32.

MEMVNKIACF VLLCMVVVAP HAEALTCGQV TSTLAPCLPY LMNRGPLGGC CGGVKGLLGQAQTTVDRQTA CTCLKSAASS FTGLDLGKAA SLPSTCSVNI PYKISPSTDC SKVQ

2. Please help him to identify the complete cds of this gene.
    Use the graphic view to explain all the features of this gene
Lycopersicon pennellii lipid transfer protein 2 (LpLTP2) gene, complete cds.
     ACCESSION   U66466
     NID         g1519356
     CDS 707..1041,1357..1366
           661 707atgg aaatggttaa
           721 caagattgca tgctttgtgc ttttatgcat ggtagtggtt gcaccccatg cagaggcact
           781 aacttgtggt caagttacat ctaccttggc tccttgtctc ccttatctaa tgaatcgcgg
           841 tcctctcgga ggctgttgtg gtggtgttaa gggtcttttg ggtcaagccc agactacagt
           901 agaccgacag accgcatgca cttgcctaaa atcagctgct tcttctttta caggccttga
           961 tttgggcaaa gctgctagtc tccctagcac ttgtagtgtc aacatccctt acaagatcag
          1021 cccctctact gactgctcta a
          1357 agtt cagtaa1366
     //
Graphical view

3.After translation of this gene, please help him to do the protein sequence analysis
(including pI, mol. wt., secondary structure prediction, hydrophobic profile,
homology search, prosite scanning..........)

Molecular weight: 11715.81
Theoretical pI: 8.36
secondary structure prediction

alpha-helix:

beta-sheet :