1. Please find the 1.9 crystal structure of the Aequorea victoria green fluorescent protein (GFP) from PDB. Give the following informations:

a.)PDB ID number.

b.)Name of the authors.
c.) Expression system used by authors.
d.) Residue number.
e.) Number of helix.
f.) The starting residue and ending residue of each helix.
g.) Number of b-sheet.

answer1-1

(a)PDB ID number: 1EMA
(b)author: M.Ormo,A.B.Cubltt,K.Kallo,L.A.Gross,R.Y.Tslen,S.J.Remlngton                        
(c)expression system: E.coli
(d)residue number: 236
(e)number of helix: 4
(f)HELIX  1   GLY  4   PHE     8                                      
   HELIX  2   TRP  57  LEU    60                                 
   HELIX  3   GLN  69  PHE    71                                      
   HELIX  4   PHE  83  SER    86                                                               
(g)beta sheet number: 11

answer1-2

(a)PDB ID number: 1GFL
(b)author: F.Yang, L.G.Moss,G.N.Philips Junion
(c)expression system: E.coli                         
(d)residue number: 238
(e)helix number: 12                                                  
(f)HELIX  1   GLU A    5  PHE A    8                                                               
   HELIX  2   TRP A   57  PHE A   64                                                             
   HELIX  3   GLN A   69  PHE A   71                                                               
   HELIX  4   ASP A   76  HIS A   81                                        
   HELIX  5   PHE A   83  SER A   86                                  
   HELIX  6   LYS A  156  LYS A  158                                      
   HELIX  7   GLU B    5  PHE B    8                                       
   HELIX  8   TRP B   57  PHE B   64                                       
   HELIX  9   GLN B   69  PHE B   71                                       
   HELIX 10   ASP B   76  HIS B   81                                       
   HELIX 11   PHE B   83  SER B   86                                      
   HELIX 12   LYS B  156  LYS B  158 
(g)beta sheet number: 12

2. Show three different Rasmol pictures of GFP:

whatever you like.

highlight the chromophore by spacefill and green.

a rotating molecule by GIF builder.

3. A blue emission variant of GFP has been made by mutation of two residues. Please find the structure of this blue emission variant. Show its structure and color the two mutated positions blue.