1.利用translate tool可得：W M P C S G G N M K K S I L F I F L S V L S F S P F A Q D A K P V E S S K E K I T L E SK K C N I A K K S N K S G P E S M N S S N Y C C E L C C N P A C T G C Y Stop Stop Y KG N Stop T V P F I F V L I L M M S V T Y V L L L C Stop I N R

2.用ncbi的blast來查為E.coli的heat stable toxin(st gene)但是score只有85％,再用swiss model submit上去所得到的homology search為sorry………….,因此判斷此為一new protein

3. 利用sps可查出positive charge 6個negative有12個

4. red:nonpolor a. a.

blue:polar uncharged a.a.

positive:yellow

negative:green

WMPCSGGNMK KSILFIFLSV LSFSPFAQDA KPVESSKEKI

TLESKKCNIA KKSNKSGPES MNSSNYCCEL CCNPACTGCY

YKGNTVPFIF VLILMMSVTY VLLLCINR

5.

.

6.

[1] PDOC00001 PS00001 ASN_GLYCOSYLATION

N-glycosylation site

Number of matches: 2

1 54-57 NKSG

2 62-65 NSSN

[2] PDOC00005 PS00005 PKC_PHOSPHO_SITE

Protein kinase C phosphorylation site

Number of matches: 3

1 35-37 SSK

2 44-46 SKK

3 53-55 SNK

[3] PDOC00006 PS00006 CK2_PHOSPHO_SITE

Casein kinase II phosphorylation site

Number of matches: 2

1 35-38 SSKE

2 56-59 SGPE

[4] PDOC00246 PS00273 ENTEROTOXIN_H_STABLE

Heat-stable enterotoxins signature

67-79 CCELCCNPACTGC

7.利用expasy的"compute pI/MW"可得pI=8.89 mw=11986.23