G F P
1. The 1.9-A resolution cristal structure of the Aequorea victoria green fluorescent protein(GFP)
The following information is from the paper ----
CRYSTAL STRUCTURE OF THE AEQUOREA VICTORIA
GREEN FLUORESCENT PROTEIN
M.ORMO, A.B.CUBITT, K.KALLIO, L.A.GROSS, R.Y.TSIEN, S.J.REMINGTON
SCIENCE V. 273 1392 1996
a) PDB ID number : 1EMA
b) Name of the authors : M.ORMO, A.B.CUBITT, K.KALLIO, L.A.GROSS,
R.Y.TSIEN, S.J.REMINGTON
c) Expression system used by authors : Escherichia Coli Jm109 (De3)
d) Residue number : 236
e) Number of helix : 4
f) The starting residue and ending residue of each helix :
HELIX 1 GLY 4 --- PHE 8
HELIX 2 TRP 57 --- LEU 60
HELIX 3 GLN 69 --- PHE 71
HELIX 4 PHE 83 --- SER 86
g) Number of sheet : 11
2. The Rasmol pictures of GFP
The GFP structure
Highlight the chromophore
A rotating molecule
3. The of GFP
The following information is from the paper ----
Blue Variant Of Green Fluorescent Protein
R.M.Wachter, B.A.King, R.Heim, K.Kallio, R.Y.Tsien, S.G.Boxer, S.J.Remington
S65, H66, And G67 of GFP Are Replaced With Iic 66, Y145f.