21% sucrose solution, 0.1 cm3
13% sorbitol solution, 20 cm3
Carbohydrase enzyme mixture, Novo Viscozyme , 0.5 cm3
Paper tissue
10 cm3 plastic syringe
1 cm3 plastic syringes, 2
Test tube
Centrifuge tube
Fine glass stirring rod
Filter funnel
Strip of nylon gauze, to plug filter funnel
Microscope slide
Cover slip
Microscope with x40 objective
Water bath set at 37oC
Bench centrifuge