osmotically-induced lysis. Students can investigate the effects of a range of different
concentrations of sucrose solutions on the appearence of the protoplasts.
1.
Cut the lettuce into pieces and put them into
sorbitol solution.
2. Incubate the tube at 37oC water
bath for 5 minutes.
3. Add 0.5 cm 3
of enzyme * then incubate for another 20 minutes.
4. Filter the tissue suspension through a funnel
plugged with gauze.
5. Spin down the protoplasts to the bottom of the tube. Carefully pour
away the
liquid from the top.
6.
Resuspend the protoplasts in 0.1 cm 3
of sucrose solution.
7. Look at the result.